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Compound, storage, time of use, protocol

Please feel free to browse the Polyplus-transfection Database to find conditions for specific product, cell line or nucleic acid type:

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jetPRIME®: DNA & siRNA transfection reagent

jetPRIME® is a proprietary cationic polymer-based molecule, synthesized at Polyplus-transfection®.

jetPRIME® forms positively charged complexes with plasmid DNA, which penetrate the cell through endocytosis. Plasmid DNA is then released from the endosomes in the cytosol via the proton sponge mechanism. Subsequent nuclear import of plasmid DNA is mainly achieved once the nuclear envelope disappears during mitosis.

jetPRIME® is a very stable molecule. jetPRIME® and its buffer should be stored at 4°C to ensure long term stability.

Experiments were conducted in house to test the stability of jetPRIME®. After 48 hours at 50°C, jetPRIME® showed a similar transfection efficiency as when stored at 4°C.

jetPRIME® can undergo an accidental freeze/thaw cycle without compromising the transfection efficiency. However, for long term storage we recommend storing the reagent at 4°C.

jetPRIME® cat#114-01 (0.1 ml) is stable for at least 6 months. Other pack sizes of jetPRIME® are stable for at least one year, when stored appropriately.

  • Where can I find product literature, such as the protocol of jetPRIME®?

You can find the protocol on the jetPRIME® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/jetprime/

All product literature is available on:

Product literature

INTERFERin®: siRNA/miRNA transfection reagent

INTERFERin® is a new generation of non-liposomal cationic amphiphile transfection reagent. It was developed for in vitro delivery of siRNA into mammalian cells grown adherent or in suspension. In many cell lines, INTERFERin® leads to more than 90% of silencing efficiency with as little as 1 nM of siRNA.

INTERFERin® should be stored tightly capped at 4°C. INTERFERin® should not be frozen, as this strongly compromises its efficiency. We recommend discarding the frozen tube and using a new one.

INTERFERin® cat#409-01 (0.1 ml) is stable for at least 6 months. Other pack sizes of INTERFERin® are stable for at least one year, when stored appropriately.

  • Where can I find product literature, such as the INTERFERin® protocol?

You can find the protocol on the INTERFERin® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/interferin/

All product literature is available on:

Product literature

jetMESSENGER®: mRNA transfection reagent

jetMESSENGER® is a cationic polymer developed by Polyplus-transfection®. This transfection reagent facilitates the entry of messenger RNA (mRNA) in mammalian cells, and allows high transfection efficiency to be reached, notably in hard to transfect cells.

jetMESSENGER® and the provided mRNA buffer should be stored at 4°C to ensure long-term stability.

Experiments were conducted in house to test the stability of jetMESSENGER®. When stored 9 months at room temperature or at -20°C, jetMESSENGER® showed a similar transfection efficiency as if stored at 4°C.

jetMESSENGER® cat#150-01 (0.1 ml) is stable for at least 6 months. Other pack sizes of jetMESSENGER® are stable for at least one year, when stored appropriately.

  • Where can I find product literature, such as the protocol of jetMESSENGER®?

You can find the protocol on the jetMESSENGER® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/jetmessenger/

All product literature is available on:

Product literature

PEIpro® & PEIpro®-HQ: From R&D to Clinical grade virus production

PEIpro® and PEIpro®-HQ are composed of a linear polyethylenimine (PEI) whose molecular weight is proprietary information. Extensive optimization experiments have been performed to select a linear PEI for optimal DNA delivery with the lowest cytotoxicity in vitro.

PEIpro® is provided as an aqueous solution of highly pure and qualified linear PEI at a concentration of 1 mg/L.

PEIpro® can undergo an accidental freeze/thaw cycle without compromising the transfection efficiency. However, for long term storage we recommend storing the reagent at 4°C to ensure long term stability.

  • How long can I use my PEIpro® vial for?

PEIpro® is stable for at least two years, when stored appropriately.

FectoPRO® & FectoCHO™ CD Medium to achieve high protein yields

FectoPRO® can undergo an accidental freeze/thaw cycle without compromising the transfection efficiency. However, for long term storage we recommend storing the reagent at 4°C to ensure long term stability.

FectoPRO® is stable for at least one year, when stored appropriately.

You can find the protocol on the FectoPRO® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/fectopro/

jetPEI®: DNA transfection reagent for HTS applications

jetPEI® is composed of a linear polyethylenimine (PEI) whose molecular weight is proprietary information. Extensive optimization experiments have been performed to select a linear PEI for optimal DNA delivery with the lowest cytotoxicity in vitro.

jetPEI® can undergo an accidental freeze/thaw cycle without compromising the transfection efficiency. However, for long term storage we recommend storing the reagent at 4°C to ensure long term stability.

  • How long can I use my jetPEI® vial for?

jetPEI® cat#101-01N (0.1 ml) is stable for 6 months. Other pack sizes of jetPEI® are stable for at least two years, when stored appropriately.

  • Where can I find product literature such as the jetPEI® protocol?

You can find the protocol on the jetPEI® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/jetpei/

All product literature is available on:

Product literature

in vivo-jetPEI® & derivatives: in vivo delivery of any nucleic acids
  • Which type of compound is in vivo-jetPEI®? What is the difference with jetPEI®?

jetPEI® (for in vitro use) and in vivo-jetPEI® are based on the same active molecule, however they have been specifically developed for each application. Thus they differ in formulation, concentration, purity and validation tests (in vivo-jetPEI® has been tested for absence of endotoxin and validated for in vivo gene delivery in mice).

in vivo-jetPEI® is very stable. However, for long-term conservation, we recommend storing in vivo-jetPEI® at -20°C upon reception.

in vivo-jetPEI® is stable for at least one year, when stored appropriately.

You can find the protocol on the in vivo-jetPEI® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/in-vivo-jetpei/

All product literature is available on:

Product literature

PULSin: Protein delivery
  • Which type of compound is PULSin?

PULSin contains a cationic amphiphile molecule capable of forming complexes with many proteins, including antibodies or peptides, resulting in overall positively charged complexes. These PULSin/proteins complexes bind to syndecans, a molecule present on virtually all cell surface membrane, allowing entry in the cell and ensuing escape of the protein from the endosome into the cytosol.

PULSin should not be frozen, as this strongly compromises its efficiency. We recommend discarding the frozen tube and using a new one.

  • How long can I use my PULSin vial for?

PULSin cat#501-01 (0.1 ml) is stable for 6 months. Other pack sizes of PULSin are stable for at least one year, when stored appropriately.

  • Where can I find product literature such the PULSin protocol?

You can find the protocol on the PULSin webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/pulsin/

All product literature is available on:

Product literature

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