INTERFERin® / jetPRIME® / in vivo-jetPEI®

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What is the most adapted product for RNA interference (gene silencing)?

INTERFERin® is the reagent of choice for siRNA transfection, and works with as little as 1 nM siRNA.

jetPRIME® is suited for siRNA delivery at higher concentrations (using 10 to 50 nM) and is recommended in case of DNA/siRNA co-delivery.

Which reagent should I use for delivery of shRNA?

shRNA can be transfected via a plasmid vector or as oligonucleotides. If shRNA are transfected as oligonucleotides, then INTERFERin® is recommended. However, if a vector expressing the shRNA is used then jetPRIME® is a more suitable reagent.

Do you have a reagent for in vivo delivery of oligonucleotides?

Our in vivo-jetPEI® is a powerful polymer-based reagent, suited for delivery of oligonucleotides to any animal model. The protocol is similar to a classical transfection: the nucleic acid and the reagent are just mixed and administered to the animal.

What reagent should I use for delivery of single-stranded oligodeoxynucleotides (ssODN)?


We recommend using jetPRIME® reagent for delivery of single-stranded oligonucleotides.

For plasmid DNA and ssODN co-transfection (e.g. CRISPR/Cas9 applications), please refer to the standard protocol we provide for DNA and siRNA co-transfection.

What reagent should I use for delivery of miRNA?

Several types of molecules are abusively called miRNA. The term miRNA can refer to:

  • mimic miRNA: these are structurally similar but not identical to siRNA and can be transfected with INTERFERin®.
  • plasmid encoding for miRNA: this molecule can be transfected using jetPRIME®.
  • antimiR: this is a single-stranded nucleic acid designed to specifically bind to and inhibit endogenous microRNA; it can be transfected using INTERFERin®.
Does the size of oligonucleotide affect the transfection efficiency?

For the same amount of transfected oligonucleotide, the number of delivered copies will be different depending on the oligonucleotide size (longer oligonucleotide: less copies, shorter oligonucleotide: more copies). Therefore, oligonucleotide amounts should be adjusted accordingly.

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