FAQ: FectoVIR®-AAV - Polyplus-transfection

Frequently asked questions about FectoVIR®-AAV

Please feel free to browse the Polyplus-transfection Database to find conditions for specific product, cell line or nucleic acid type:

Consult our database

For more information contact us directly at support@polyplus-transfection.com:

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What is the chemical structure of FectoVIR®-AAV? What is the difference with PEIpro®?

The FectoVIR^®-AAV structure is confidential and a patent has been filled. PEIpro^® and FectoVIR^®-AAV are different both in terms of molecular structure and formulation.

Where can I find optimized conditions using FectoVIR®-AAV?

You will find starting conditions as well as a range for optimization in the dedicated protocol available in the documentation tab of the FectoVIR^®-AAV webpage: https://www.polyplus-transfection.com/products/fectovir-aav/. If you need further information, please contact our scientific support using our contact us form.

In which cell types FectoVIR®-AAV has been tested?

We have successfully used FectoVIR^®-AAV on suspension HEK-293 derivatives (293T, 293F) and high density derivatives (Expi293™ cells). Beta-testers confirmed that FectoVIR^®-AAV works well in several commercial and proprietary suspension HEK-293 subclones.

Which serotypes has been produced with FectoVIR®-AAV?

In house, we produce both AAV-2 and AAV-5. More serotypes have been tested by our beta-testers, with comparable yield improvements.

Does FectoVIR®-AAV work in adherent cell culture?

FectoVIR^®-AAV shows a remarquable improvement in terms of viral titers in suspension system and is less efficient in adherent systems for which PEIpro^® remains the transfection reagent of choice.

Can I use FectoVIR®-AAV to produce recombinant lentiviruses or other types of virus?

For lentivirus production, we recommend PEIpro^® the gold standard for virus production as it is currently the best product for this application, both in adherent and suspension cell culture systems.

Is there a residual test available yet?

A residual test assay has been developed. Contact us for more information.

When will GMP grade FectoVIR®-AAV be available?

FectoVIR^®-AAV GMP will be available in Q4 2021.

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