FAQ: Protein production

FectoPRO® is the reagent of choice for biomanufacturing of proteins as it has been developed for scientists interested in obtaining best possible results with mammalian Transient Gene Expression systems. FectoPRO® ensures highest transfection efficiencies and protein yields in CHO and HEK-293 cell lines grown in variety of media. Moreover FectoPRO® shows great cell viability, reproducibility and consistency in results. FectoPRO® outperforms any other commercially available reagent and allows to optimize mammalian bioproduction processes (whatever scale used) as it requires equally less DNA and reagent.

For large or very-large scale experiments, you might want to consider PEIpro® as it is also very efficient in HEK-293 cells and cost-effective.

FectoPRO® can be used without FectoPRO® booster, but it is recommended if you want to achieve high protein yields using low DNA amounts.

FectoPRO® works beautifully in regular and high cell density systems. FectoPRO® allows to reach higher yields in a shorter timeframe – No need to wait for 7 or 12 days of cell growth to produce recombinant proteins.

The optimal DNA amount, mentioned in the FectoCHO™ Expression system protocol, is 0.8 µg DNA per mL of cell culture. If you use FectoPRO® with any other media, you still can use 0.8 µg DNA per mL of cell culture.
However, it should be noted that with the synergistic association of the FectoCHO™ CD Expression medium and the FectoPRO® powerful transfection reagent you will obtain better protein production yields. For more informations and datas please visit our FectoCHO™ Expression system page.

Both FectoPRO® and PEIpro® has been shown to work beautifully with HEK293 cells and serum-free media. But it depends on what your application is: FectoPRO® is used for protein production whereas PEIpro® is used for virus production.

No, you don’t need to buy the medium first and the transfection reagent in a second time since the FectoCHO™ Expression system kit is perfectly suited to both adapt your cells to FectoCHO™ CD Expression medium and perform transfection using FectoPRO® transfection reagent. Indeed, in the 6L FectoCHO™ Expression system kit (ref# 716-06LKIT), we provide you with 6L of medium and 5mL of transfection reagent, a perfect ratio of both reagents to allow you to conduct a full experiment from cell adaptation to protein production. Feel free to contact us for any pricing inquiry.

Depending on the medium used, 1 mL FectoPRO® is sufficient to transfect between 500mL and 1L of culture.

The DNA amount per mL is the same if you use either the FectoCHO™ expression system or the ExpiCHO™ expression system in ExpiCHO™ cells. However keep in mind that, with FectoCHO™, you can obtain up to twice the protein production yield at a lower cost.