Protocol
- Description: Human acute monocytic leukaemia cells
Known as: THP1, THP 1 - Delivered Biomolecule: mRNA
Transfection Reagent | jetMESSENGER |
---|---|
Plate format | 24-well plate |
Cell culture conditions | RPMI 1640, 10% FBS, 2mM glutamine, 1% P/S, 1% HEPES, 1% Sodium pyruvate, 0,05mM beta-mercaptoethanol |
Transfection medium | Same as cell culture medium |
Number of seeded cells/Confluency | Suspension cells - Density: 100 000 cells/well |
Transfection volume | 0.5 mL |
Amount of biomolecule | 500 ng |
Amount of reagent | 1 µL |
Transfection analysis performed after | 24h |