• Description: Human primary melanocyte cells
  • Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
Transfection ReagentjetCRISPR, SpCas9 Nuclease
Plate format96-well plate
Cell culture conditions

Melanocytes Growth medium M2

Transfection mediumReduced serum medium followed by a medium change 4h post-transfection with a complete medium (same as above)
Number of seeded cells/ConfluencyForward: 7 500 cells/well
Transfection volume125 µL
Amount of biomolecule30 nM
Amount of reagent0.3 µL
Transfection analysis performed after48h