• Description: Primary human hepatocytes
  • Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
Transfection ReagentjetCRISPR, SpCas9 Nuclease
Plate format96-well plate
Cell culture conditions

Hepatocyte Maint. Media w/ Supplement (ref: MM250, Lonza)

Transfection mediumSame as above
Number of seeded cells/ConfluencyForward protocol: 40 000 cells/ well
Transfection volume150 µL
Amount of biomolecule30 nM
Amount of reagent0.3 µL
Transfection analysis performed after48 h