Protocol
- Description: Primary human fibroblast cells
- Delivered Biomolecule: mRNA
| Transfection Reagent | jetMESSENGER |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | Fibroblast Basal Medium, 2% FBS, 0,1% insuline, 0,1% rhFGF, 0,1% Gentamycine-sulfate |
| Transfection medium | Same as cell culture medium |
| Number of seeded cells/Confluency | 60-80% |
| Transfection volume | 0.5 mL |
| Amount of biomolecule | 500 ng |
| Amount of reagent | 1.2 µL |
| Transfection analysis performed after | 24h |