Protocol
- Description: Human breast adenocarcinoma cells
Known as: MCF-7, MCF 7 - Delivered Biomolecule: mRNA
Transfection Reagent | jetMESSENGER |
---|---|
Plate format | 24-well plate |
Cell culture conditions | MEM with Earl's salt, 10% FBS, 2mM glutamine, 2% P/S, 1% AANE |
Transfection medium | Same as cell culture medium |
Number of seeded cells/Confluency | 60-80% |
Transfection volume | 0.5 mL |
Amount of biomolecule | 500ng |
Amount of reagent | 1µL |
Transfection analysis performed after | 24h |