Protocol
- Description: Human breast adenocarcinoma cells
Known as: MCF10A, MCF 10A - Delivered Biomolecule: DNA
Transfection Reagent | jetOPTIMUS |
---|---|
Plate format | 24 well-plate |
Cell culture conditions | MEBM + MEGM SingleQuot additives + 100 ng/mL cholera toxin |
Transfection medium | Complete Medium followed by a medium change 4h post-transfection with complete medium |
Number of seeded cells/Confluency | 80 000 cells |
Transfection volume | 500 µL |
Amount of biomolecule | 500 ng |
Amount of reagent | 0.75 µL |
Transfection analysis performed after | 24h |