Protocol
- Description: Human breast adenocarcinoma cells
Known as: MCF10A, MCF 10A - Delivered Biomolecule: mRNA
| Transfection Reagent | jetMESSENGER |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | MEBM, 0,4% BPE, 0,1% hEGF, 0,1% hydrocortisone, 0,1% gentamycine, 0,1% insuline, 100ng/mL cholera toxin |
| Transfection medium | Same as cell culture medium |
| Number of seeded cells/Confluency | 60-80% |
| Transfection volume | 0.5 mL |
| Amount of biomolecule | 500 ng |
| Amount of reagent | 1.2 µL |
| Transfection analysis performed after | 24h |