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Protocol

  • Description: Primary human keratinocytes
    Known as: NHEK
  • Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
Transfection Reagent jetCRISPR, SpCas9 Nuclease
Plate format 96-well plate
Cell culture conditions

Keratinocytes Growth medium 2

Transfection medium Reduced serum medium followed by a medium change 4h post-transfection with a complete medium (same as above)
Number of seeded cells/Confluency Forward: 5 000 cells/well
Transfection volume 125 µL
Amount of biomolecule 30 nM
Amount of reagent 0.3 µL
Transfection analysis performed after 48h