Home > Resources > Polyplus-transfection Database > Primary keratinocytes: 10-30 % of INDEL at 30 nM RNP
  • Description: Primary human keratinocytes
    Known as: NHEK
  • Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
Transfection ReagentjetCRISPR, SpCas9 Nuclease
Plate format96-well plate
Cell culture conditions

Keratinocytes Growth medium 2

Transfection mediumReduced serum medium followed by a medium change 4h post-transfection with a complete medium (same as above)
Number of seeded cells/ConfluencyForward: 5 000 cells/well
Transfection volume125 µL
Amount of biomolecule30 nM
Amount of reagent0.3 µL
Transfection analysis performed after48h
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