• Description: Human umbilical vein endothelial cells
  • Delivered Biomolecule: DNA
Transfection ReagentjetOPTIMUS
Plate format24-well plate
Cell culture conditions

Endothelial cell growth medium + Supplement mix (Fetal calf serum, Endothelial cell growth supplement, Epidermal Growth FactorBasic Fibroblast Growth Factor, Heparin, Hydrocortisone)

Transfection mediumBasic Medium followed by a medium change 4h post-transfection with complete medium
Number of seeded cells/Confluency20 000 cells
Transfection volume500 µL
Amount of biomolecule500 ng
Amount of reagent0.75 µL
Transfection analysis performed after24h