Protocol
- Description: Human umbilical vein endothelial cells
- Delivered Biomolecule: DNA
Transfection Reagent | jetOPTIMUS |
---|---|
Plate format | 24-well plate |
Cell culture conditions | Endothelial cell growth medium + Supplement mix (Fetal calf serum, Endothelial cell growth supplement, Epidermal Growth FactorBasic Fibroblast Growth Factor, Heparin, Hydrocortisone) |
Transfection medium | Basic Medium followed by a medium change 4h post-transfection with complete medium |
Number of seeded cells/Confluency | 20 000 cells |
Transfection volume | 500 µL |
Amount of biomolecule | 500 ng |
Amount of reagent | 0.75 µL |
Transfection analysis performed after | 24h |