Protocol
- Description: Primary human hepatocytes
- Delivered Biomolecule: siRNA
| Transfection Reagent | INTERFERin |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | Hepatocyte Complete Medium |
| Transfection medium | Hepatocyte Basal Medium (without antibiotics and serum) |
| Number of seeded cells/Confluency | 30 - 50% |
| Transfection volume | 0.5 ml |
| Amount of biomolecule | 1 nM |
| Amount of reagent | 2 µl |
| Transfection analysis performed after | 48 h |