• Description: Primary rat hippocampal neurons
  • Delivered Biomolecule: mRNA
Transfection ReagentjetMESSENGER
Plate format48-well plate
Cell culture conditions

Neurobasal medium, 2% B27 50x, Glutamate 25µM

Transfection mediumSame as cell culture medium
Number of seeded cells/ConfluencyMorphology : Cortex Neurons must be mature enough to develop dendrites - Density: 100 000 cells/well
Transfection volume0.5 mL
Amount of biomolecule150 ng
Amount of reagent0,3 µl
Transfection analysis performed after24 - 48h