Protocol
- Description: Human hepatocarcinoma cells
- Delivered Biomolecule: DNA
| Transfection Reagent | jetOPTIMUS |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | EMEM + 10% FBS + 2% Pen/Strep + 1% Glutamine + 1% AANE + 1% Sodium Pyruvate |
| Transfection medium | Complete Medium followed by a medium change 4h post-transfection with complete medium |
| Number of seeded cells/Confluency | 100 000 cells |
| Transfection volume | 500 µL |
| Amount of biomolecule | 500 ng |
| Amount of reagent | 0.75 µL |
| Transfection analysis performed after | 24h |