Protocol
- Description: Human hepatocarcinoma cells
- Delivered Biomolecule: siRNA
Transfection Reagent | INTERFERin |
---|---|
Plate format | 24-well plate |
Cell culture conditions | EMEM with 2 mM glutamine, 1.5 g/l sodium bicarbonate, 0.1 mM NEAA, 0.1 mM sodium pyruvate, antibiotics, 10% FBS |
Transfection medium | Same as above |
Number of seeded cells/Confluency | 30 - 50% |
Transfection volume | 0.5 ml |
Amount of biomolecule | 10 nM |
Amount of reagent | 2 µl |
Transfection analysis performed after | 48 h |