Protocol
- Description: Human hepatocarcinoma cells
- Delivered Biomolecule: DNA
| Transfection Reagent | jetPEI-Hepatocyte |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | EMEM with 2 mM glutamine, 1.5 g/l sodium bicarbonate, 0.1 mM NEAA, 0.1 mM sodium pyruvate, antibiotics, 10% FBS |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 60 – 70% |
| Transfection volume | 1 ml |
| Amount of biomolecule | 2 µg |
| Amount of reagent | 6.4 µl |
| Transfection analysis performed after | 24 h |