Protocol

  • Description: Human cervix epitheloid carcinoma cells
  • Delivered Biomolecule: DNA
Transfection Reagent jetOPTIMUS
Plate format 24 well-plate
Cell culture conditions

EMEM + 10% FBS + 2% Pen/Strep + 1% Glutamine + 1% AANE

Transfection medium Complete Medium followed by a medium change 4h post-transfection with complete medium
Number of seeded cells/Confluency 50 000
Transfection volume 500 µL
Amount of biomolecule 500 ng
Amount of reagent 0.5 µL
Transfection analysis performed after 24h