Protocol

  • Description: Human cervix epitheloid carcinoma cells
  • Delivered Biomolecule: siRNA
Transfection Reagent INTERFERin
Plate format 24-well plate
Cell culture conditions MEM with Earle's salt, 2 mM glutamine, antibiotics, 10% FBS
Transfection medium Same as above
Number of seeded cells/Confluency 30 - 50%
Transfection volume 0.5 ml
Amount of biomolecule 1 nM
Amount of reagent 2 µl
Transfection analysis performed after 48 h