Protocol
- Description: Human cervix epitheloid carcinoma cells
- Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
| Transfection Reagent | jetCRISPR, SpCas9 Nuclease |
|---|---|
| Plate format | 96-well plate |
| Cell culture conditions | MEM + 1% NEAA (non-essential amino acids) + 10% FBS + 1% Glutamine + 2% Pen/Strep |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | Reverse: 20 000 cells/well or Forward: 12 000 cells/well |
| Transfection volume | 125 µL |
| Amount of biomolecule | 30 nM |
| Amount of reagent | 0.3 µL |
| Transfection analysis performed after | 48 h |