• Description: Human embryonic kidney Fibroblast
    Known as: HEK293, 293
  • Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
Transfection ReagentjetCRISPR, SpCas9 Nuclease
Plate format96-well plate
Cell culture conditions

Pre-coated plate with 50µL fibronectine at 0.05 mg/mL and use a MEM culture medium + 1% NEAA (non-essential amino acids) + 10% FBS + 1% Glutamine + 2% Pen/Strep

Transfection mediumSame as above
Number of seeded cells/ConfluencyReverse: 20 000 cells/well or Forward: 12 000 cells/well
Transfection volume125 µL
Amount of biomolecule30 nM
Amount of reagent0.3 µL
Transfection analysis performed after48 h