Protocol
- Description: Human embryonic kidney Fibroblast
Known as: FreeStyle 293-F, HEK293-F, 293-F - Delivered Biomolecule: DNA
Transfection Reagent | PEIpro |
---|---|
Plate format | shaker flask |
Cell culture conditions | FreeStyle™ F17 (Life Technologies™), FreeStyle™ 293 (Life Technologies™), HyClone™ HyCell™ TransFx™-H (GE Healthcare), Pro293™ (Lonza) |
Transfection medium | Same as above |
Number of seeded cells/Confluency | 1 x 10e6/ ml one day before transfection |
Transfection volume | Contact Tech Support for more details |
Amount of biomolecule | Contact Tech Support for more details |
Amount of reagent | Contact Tech Support for more details |
Transfection analysis performed after | - |