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Protocol

  • Description: Human embryonic kidney Fibroblast
    Known as: FreeStyle 293-F, HEK293-F, 293-F
  • Delivered Biomolecule: DNA
Transfection Reagent PEIpro
Plate format shaker flask
Cell culture conditions FreeStyle™ F17 (Life Technologies™), FreeStyle™ 293 (Life Technologies™), HyClone™ HyCell™ TransFx™-H (GE Healthcare), Pro293™ (Lonza)
Transfection medium Same as above
Number of seeded cells/Confluency 1 x 10e6/ ml one day before transfection
Transfection volume Contact Tech Support for more details
Amount of biomolecule Contact Tech Support for more details
Amount of reagent Contact Tech Support for more details
Transfection analysis performed after -