Protocol
- Description: Human embryonic kidney Fibroblast
Known as: HEK293, 293 - Delivered Biomolecule: siRNA
| Transfection Reagent | INTERFERin |
|---|---|
| Plate format | 96-well plate |
| Cell culture conditions | DMEM with 2 mM glutamine, antibiotics, 10% FBS |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 10 000 cells per well |
| Transfection volume | 125 µl |
| Amount of biomolecule | 1 nM |
| Amount of reagent | 0.75 µl |
| Transfection analysis performed after | 48 h |