Protocol
- Description: Human embryonic kidney Fibroblast
Known as: HEK293, 293 - Delivered Biomolecule: DNA
| Transfection Reagent | FectoVIR-AAV |
|---|---|
| Plate format | 125 mL shake flask |
| Cell culture conditions | Complexation medium : DMEM 1 g/L; complexation volume: 5% |
| Transfection medium | Freestyle F17 |
| Number of seeded cells/Confluency | 2.10e6 (day of transfection) |
| Transfection volume | 125 mL |
| Amount of biomolecule | 1 µg / 10e6 cells |
| Amount of reagent | 1 µL / µg DNA |
| Transfection analysis performed after | 72h |