Protocol
- Description: Human colon carcinoma cells
Known as: HCT116 - Delivered Biomolecule: siRNA
Transfection Reagent | INTERFERin |
---|---|
Plate format | 24-well plate |
Cell culture conditions | DMEM with 4.5 g/L glucose, 50 mM Hepes, 2 mM glutamine, antibiotics, 10% FBS |
Transfection medium | Same as above |
Number of seeded cells/Confluency | 30 - 50% |
Transfection volume | 0.5 ml |
Amount of biomolecule | 10 nM |
Amount of reagent | 1 µl |
Transfection analysis performed after | 48 h |