Protocol
- Description: Human monocyte derived dendritic cells
Known as: DC - Delivered Biomolecule: mRNA
| Transfection Reagent | jetMESSENGER |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | 7 days before transfection, primary monocytes are seeded at 200 000-300 000 cells/well in a complete medium RPMI. Monocytes are differentiated by adding 200U of IL-4+500U of GMCSF. Change the medium every 2 days. |
| Transfection medium | RPMI (complete medium) + IL-4 + GMCSF |
| Number of seeded cells/Confluency | - |
| Transfection volume | 0.5 mL |
| Amount of biomolecule | 500 ng |
| Amount of reagent | 1 µL |
| Transfection analysis performed after | 24 h |