Home > Resources > Polyplus-transfection Database > CPRE-2: 40-60% transfection efficiency
  • Description: Primary human fibroblast cells
  • Delivered Biomolecule: DNA
Transfection ReagentjetOPTIMUS
Plate format24-well plate
Cell culture conditions

DMEM 1g/L glucose + 10% FBS + 1% Pen/Strep + 1% AANE + 1% Sodium Pyruvate + 1% Glutamax

Transfection mediumComplete Medium followed by a medium change 4h post-transfection with complete medium
Number of seeded cells/Confluency40 000 cells
Transfection volume500 µL
Amount of biomolecule500 ng
Amount of reagent0.5 µL
Transfection analysis performed after24h
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