Protocol
- Description: Primary human fibroblast cells
- Delivered Biomolecule: DNA
| Transfection Reagent | jetOPTIMUS |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | DMEM 1g/L glucose + 10% FBS + 1% Pen/Strep + 1% AANE + 1% Sodium Pyruvate + 1% Glutamax |
| Transfection medium | Complete Medium followed by a medium change 4h post-transfection with complete medium |
| Number of seeded cells/Confluency | 40 000 cells |
| Transfection volume | 500 µL |
| Amount of biomolecule | 500 ng |
| Amount of reagent | 0.5 µL |
| Transfection analysis performed after | 24h |