Protocol
- Description: Chinese hamster ovary cells
- Delivered Biomolecule: siRNA
Transfection Reagent | INTERFERin |
---|---|
Plate format | 96-well plate |
Cell culture conditions | HAM F12 with 2 mM glutamine, antibiotics, 10% FBS |
Transfection medium | Same as above |
Number of seeded cells/Confluency | 10 000 cells per well |
Transfection volume | 125 µl |
Amount of biomolecule | 10 nM |
Amount of reagent | 0.75 µl |
Transfection analysis performed after | 48 h |