Protocol
- Description: Human colon carcinoma epithelial cells
- Delivered Biomolecule: mRNA
Transfection Reagent | jetMESSENGER |
---|---|
Plate format | 24-well plate |
Cell culture conditions | DMEM 4,5g/L glucose, 20% FBS, 1% AANE, 1% NaPy, 2mM glutamine, 2% P/S |
Transfection medium | Same as cell culture medium |
Number of seeded cells/Confluency | 60-80% |
Transfection volume | 0.5 mL |
Amount of biomolecule | 500 ng |
Amount of reagent | 1.2 µL |
Transfection analysis performed after | 24h |