Protocol
- Description: Human colon carcinoma epithelial cells
- Delivered Biomolecule: DNA
Transfection Reagent | jetPEI |
---|---|
Plate format | 24-well plate |
Cell culture conditions | DMEM with 4.5 g/l glucose, 1% Sodium Pyruvate, 1% NEAA, 2 mM glutamine, antibiotics, 20% FBS |
Transfection medium | Same as above |
Number of seeded cells/Confluency | 60 – 70% |
Transfection volume | 0.5 ml |
Amount of biomolecule | 1 µg |
Amount of reagent | 2 µl |
Transfection analysis performed after | 24 h |