• Description: Human colon carcinoma epithelial cells
  • Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
Transfection ReagentjetCRISPR, SpCas9 Nuclease
Plate format96-well plate
Cell culture conditions

DMEM 4,5 g/L glucose + 1% AANE + 1% Na Pyruvate + 20% FBS + 1% Glutamine + 2% Pen / Strep

Transfection mediumSame as Above
Number of seeded cells/ConfluencyReverse transfection: 20 000 cells/well
Transfection volume125 µL
Amount of biomolecule30 nM
Amount of reagent0.3 µL
Transfection analysis performed after48 h