• Description: Mouse embryonic fibroblastic cells
    Known as: 10T1/2
  • Delivered Biomolecule: DNA
Transfection ReagentjetPRIME
Plate format6-well plate
Cell culture conditionsDMEM with 4.5 g/L glucose, 50 mM Hepes, 2 mM glutamine, antibiotics, 10% FBS
Transfection mediumDMEM 4.5 g/l glucose, 1% non-essential amino-acids, 2 mM glutamine, antibiotics, 10% FBS
Number of seeded cells/Confluency200 000 cells
Transfection volume0.5 ml
Amount of biomolecule2 µg
Amount of reagent5 µl
Transfection analysis performed afternot specified