Protocol

  • Description: Murine myoblasts
  • Delivered Biomolecule: DNA
Transfection Reagent jetPRIME
Plate format 24-well plate
Cell culture conditions DMEM 4.5 g/l glucose, 1% non-essential amino-acids, 2 mM glutamine, antibiotics, 10% FBS
Transfection medium Same as above
Number of seeded cells/Confluency 40 000 cells per well
Transfection volume 0.5 ml
Amount of biomolecule 0.75 µg
Amount of reagent 1.5 µl
Transfection analysis performed after 24 h