Protocol
- Description: Murine normal embryonic hepatocytes
- Delivered Biomolecule: DNA
| Transfection Reagent | jetPEI-Hepatocyte |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | DMEM 4.5 g/l glucose, 2 mM glutamine, antibiotics, 10% FBS |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 60 – 70% |
| Transfection volume | 1 ml |
| Amount of biomolecule | 1 µg |
| Amount of reagent | 2 µl |
| Transfection analysis performed after | 24 h |