• Description: Murine normal embryonic hepatocytes
  • Delivered Biomolecule: DNA
Transfection ReagentjetPRIME
Plate format24-well plate
Cell culture conditionsDMEM 4.5 g/l glucose, 2 mM glutamine, antibiotics, 10% FBS Gently centrifuge the culture plates for 5 min at 280g after adding DNA/jetPRIME complexes to the cells
Transfection mediumSame as above
Number of seeded cells/Confluency60 – 70%
Transfection volume1 ml
Amount of biomolecule0,5 µg
Amount of reagent2 µl
Transfection analysis performed after24 h