Protocol
- Description: Human lung carcinoma cells, type II pneumocytes
Known as: A-549 - Delivered Biomolecule: Protein/Peptide/Antibody
| Transfection Reagent | PULSin |
|---|---|
| Plate format | 12- well plate |
| Cell culture conditions | DMEM with 2 mM glutamine, antibiotics, 10% FBS |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 30 - 50 % |
| Transfection volume | 0.5 ml |
| Amount of biomolecule | 40 nM |
| Amount of reagent | 6 µl |
| Transfection analysis performed after | - |