Home > Resources > Polyplus-transfection Database > A549: 40-60% transfection efficiency
  • Description: Human lung carcinoma cells, type II pneumocytes
    Known as: A-549
  • Delivered Biomolecule: DNA
Transfection ReagentjetOPTIMUS
Plate format24-well plate
Cell culture conditions

Ham's F12 + 10% FBS + 2% Pen/Strep + 1% Glutamine

Transfection mediumComplete Medium followed by a medium change 4h post-transfection with complete medium
Number of seeded cells/Confluency60 000 cells
Transfection volume500 µL
Amount of biomolecule500 ng
Amount of reagent0.5 µL
Transfection analysis performed after24h
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