• Description: Human lung carcinoma cells, type II pneumocytes
    Known as: A-549
  • Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
Transfection ReagentjetCRISPR, SpCas9 Nuclease
Plate format96-well plate
Cell culture conditions

MEM + 1% NEAA (non-essential amino acids) + 10% FBS + 1% Glutamine + 2% Pen/Strep

Transfection mediumSame as above
Number of seeded cells/ConfluencyReverse: 20 000 cells/well or Forward: 12 000 cells/well
Transfection volume125 µL
Amount of biomolecule30 nM
Amount of reagent0.3 µL
Transfection analysis performed after48 h