Protocol
- Description: Mouse stem cells derived from preimplantation-stage embryo.
Known as: mESCs - Delivered Biomolecule: mRNA
| Transfection Reagent | jetMESSENGER |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | DMEM 4,5g/L glucose (250mL), 15% FBS, 2mM glutamine, 1% AANE, 1% P/S, 1% HEPES, 3,4µL monothioglycerol, ESGROmLIF (25µL ESG1107 + 250µL ESG1106) |
| Transfection medium | Same as cell culture medium |
| Number of seeded cells/Confluency | Differentiation must be advanced enough for cells to form colonies |
| Transfection volume | 0.5 mL |
| Amount of biomolecule | 500 ng |
| Amount of reagent | 1 µl |
| Transfection analysis performed after | 24h |