Home > Resources > Polyplus-transfection Database > mES: 50-70% transfection efficiency
  • Description: Mouse stem cells derived from preimplantation-stage embryo. 
    Known as: mESCs
  • Delivered Biomolecule: mRNA
Transfection ReagentjetMESSENGER
Plate format24-well plate
Cell culture conditions

DMEM 4,5g/L glucose (250mL), 15% FBS, 2mM glutamine, 1% AANE, 1% P/S, 1% HEPES, 3,4µL monothioglycerol, ESGROmLIF (25µL ESG1107 + 250µL ESG1106)

Transfection mediumSame as cell culture medium
Number of seeded cells/ConfluencyDifferentiation must be advanced enough for cells to form colonies
Transfection volume0.5 mL
Amount of biomolecule500 ng
Amount of reagent1 µl
Transfection analysis performed after24h
 Back