Protocol
- Description: Murine Lewis lung carcinoma cells
- Delivered Biomolecule: siRNA
| Transfection Reagent | INTERFERin |
|---|---|
| Plate format | 12-well plate |
| Cell culture conditions | DMEM containing 4.5 g/l glucose, 2 mM glutamine, antibiotics, 10% fetal calf serum |
| Transfection medium | - |
| Number of seeded cells/Confluency | 150 000 cells per well |
| Transfection volume | 1 ml |
| Amount of biomolecule | 1 nM |
| Amount of reagent | 3 µl |
| Transfection analysis performed after | 48 h |