jetSI 10 mM

Molecule delivered



in vivo siRNA transfection

Targeted organs


Injection route

Intracranial injection
Stereotaxical injection
Intraventricular injection

Number of transfections

500 µl of jetSI 10 mM delivery reagent is sufficient to deliver up to 2.5 mg of siRNA to mouse brain (c.a. 100 ng of siRNA per injection).


5°C ± 3°C, for at least 6 months


in vivo-jetPEI® is broadly used as an in vivo transfection reagent. However, for siRNA delivery to the brain, Polyplus-transfection® has developed jetSI 10 mM.

The choice of the most effective siRNA carrier may depend on the in vivo context. Indeed, while in vivo-jetPEI® is superior to cationic liposomes for plasmid DNA delivery in the mouse brain, jetSI 10 mM was found to be the carrier of choice for siRNA delivery to this organ (Froidevaux et al. (2006), EMBO Rep 7:1035; Guissouma et al. (2006), Neurosci Lett 406: 240; Kumar et al. (2006), PLOS Med. 3: 0505; Hassani et al. (2005), J Gene Med 7:198).

When using plasmid based approaches (shRNA) in the brain, we recommend in vivo-jetPEI®.

Ordering information

Reference NumberAmount of reagent
403-050.5 ml


in vivo functional studies

jetSI 10 mM is perfectly suited to study gene function in the brain in vivo and provides a useful method for the validation of in vitro functional studies into animals.


Polyplus-transfection® is ISO 9001 Quality Management System accredited since 2002; this level of certification assures global customers that the supplier has established reliable and effective processes for product development, manufacturing, sales and customer support.


« I finished the experiments and I think in vivo-jetPEI works great. I am very pleased with the results. Actually some of the work we have done with in vivo-jetPEI® as well as jetSI 10 mM has been published in Science. I would like to tell you that I am also very pleased with the technical assistance. Many thanks! »
 E.E.K., Hacettepe University, Turkey 


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Other files


Order by :  
Found 17 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
-in vivosiRNAjetSI 10 mM
Bender, A. C. et al. (2013)

Neurobiol Dis 54, 297-307
Focal Scn1a knockdown induces cognitive impairment without seizures
More details
-in vivosiRNAjetSI 10 mM
Cakir, I. et al. (2009)

PLoS One 4, e8322
Hypothalamic Sirt1 regulates food intake in a rodent model system
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-in vivosiRNAjetSI 10 mM
Carlsson, Y. et al. (2011)

Ann Neurol 70, 781-9
Genetic inhibition of caspase-2 reduces hypoxic-ischemic and excitotoxic neonatal brain injury
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-in vivosiRNAjetSI 10 mM
Chauvier, D. et al. (2011)

Cell Death Dis 2, e203
Targeting neonatal ischemic brain injury with a pentapeptide-based irreversible caspase inhibitor
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-in vivosiRNAjetSI 10 mM
Cheret, C. et al. (2008)

J Neurosci 28, 12039-51
Neurotoxic activation of microglia is promoted by a nox1-dependent NADPH oxidase
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-in vivosiRNAjetSI 10 mM
Dominska, M. et al. (2010)

J Cell Sci 123, 1183-9
Breaking down the barriers: siRNA delivery and endosome escape
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-in vivoDNA, siRNAjetSI 10 mM
Froidevaux, M. S. et al. (2006)

EMBO Rep 7, 1182
The co-chaperone XAP2 is required for activation of hypothalamic thyrotropin-releasing hormone transcription in vivo
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-in vivomimic miRNAjetSI 10 mM
Griggs, E. M. et al. (2013)

J Neurosci 33, 1734-40
MicroRNA-182 regulates amygdala-dependent memory formation
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-in vivoDNA, siRNAjetSI 10 mM
Guissouma, H. et al. (2006)

Neurosci Lett 406, 240-3
In vivo siRNA delivery to the mouse hypothalamus confirms distinct roles of TR beta isoforms in regulating TRH transcription
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-in vivoDNA, siRNAjetSI 10 mM
Hassani, Z. et al. (2005)

J Gene Med 7, 198-207
Lipid-mediated siRNA delivery down-regulates exogenous gene expression in the mouse brain at picomolar levels
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