Overview
Specifications
| Reagent | jetPRIME® |
|---|---|
| Molecule delivered | DNA, siRNA, DNA & siRNA |
| Applications | Plasmid transfection |
| Cell types | Adherent cell lines grown in presence of serum |
| Number of transfections | 1.5 ml of jetPRIME® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates |
| Storage | 5°C ± 3°C, stable for at least one year when stored appropriately |
| Provided with | jetPRIME buffer |
Summary
jetPRIME® is a powerful and versatile DNA and siRNA transfection reagent for day-to-day experiments that leads to efficient and reliable scientific results. jetPRIME® ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. jetPRIME ® is also ideal for DNA/siRNA co-transfection or co-delivery of several plasmids. Furthermore, our jetPRIME® reagent is very gentle on cells since it requires low amounts of reagent and nucleic acid during transfection.
Ordering information
| Reference Number | Amount of reagent | Amount of buffer |
|---|---|---|
| 114-01 | 0.1 ml | 5 ml |
| 114-07 | 0.75 ml | 60 ml |
| 114-15 | 1.5 ml | 2 x 60 ml |
| 114-75 | 5 x 1.5 ml | 10 x 60 ml |
| 712-60 | x | 60mL |
Description
Superior transfection efficiency
jetPRIME® is a powerful transfection reagent for day-to-day experiments. It leads to unusually high percentage of transfected adherent cell lines of various origins as well as primary cells.
Superior transfection efficiencies ranging between 70 and 90% were obtained with jetPRIME® reagent versus the top competitor’s reagent for several commonly used cell lines (Fig.1 & Fig.2).
Fig. 1: Comparative transfection efficiency of jetPRIME versus its main competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection in 24-well plates. Conditions were used according to the manufacturer’s recommendation for both Lipofectamine® 2000 and for jetPRIME®.
Fig. 2: Comparative transfection efficiency of jetPRIME® versus Lipofectamine® 3000. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection in 96-well plates or 24-well plates. Conditions were used according to the manufacturer’s recommendation for lipofectamine® 3000 and for jetPRIME®.
Cost-effective: less DNA and less reagent
jetPRIME® is a powerful in vitro transfection reagent that requires a small amount of reagent and plasmid DNA, making its use very cost-effective (Table 1).
Table 1: Recommended conditions to use. Amounts of DNA and reagent (jetPRIME® and competitors) per well in 6-well plate for transfection according to manufacturers’ recommendations.
In addition to reducing costs, using less DNA also minimizes adverse cytotoxic effects triggered by transfection. Hence, jetPRIME® is the reagent of choice for high transfection efficiency with excellent cell viability.
Better cell viability
jetPRIME® is extremely gentle on cells during transfection leading to increased cell viability and improved transfection results. Cells transfected with jetPRIME® are healthy, while major cytotoxicity is observed with competitors (Fig.3).
Fig. 3: Cell viability 24h after transfection. Phase contrast microscopy of HeLa cells 24 h after transfections performed according to the manufacturer’s recommendations for each reagent.
Easy to use protocol
jetPRIME® is an easy-to-use transfection reagent (Fig.4):
- Fast and easy to scale up and down
- Compatible with serum and antibiotics
Fig. 4: jetPRIME® protocol. Convenient protocol for DNA, siRNA and co-transfection of DNA and siRNA.
Take a look at our Expert Tips to ace DNA transfection in our blog section.
FAQ
If you have any questions regarding jetPRIME®, please visit our dedicated Frequently asked questions or contact us.
Applications
Plasmid transfection
jetPRIME® leads to remarkably high percentages of transfected adherent cell lines of various origin, as well as primary cells (Table 1).
Table 1: Transfection efficiency of various cell types using jetPRIME®. The percentage of GFP-positive cells was determined by FACS analysis 24 h after transfection.
Plasmids are small circular DNA molecules that are commonly found in bacteria. Plasmids exist and replicate separately from chromosomal DNA and in bacteria they often carry genes that are beneficial for bacterial survival. Plasmids can be deliberately introduced into desired cells and utilized to overexpress a gene of interest in a specific cell line. This procedure is called DNA transfection and is a commonly used method for studying gene function or protein of interest.
Genome editing
The use of the CRISPR/Cas9 system in mammalian cells has recently emerged as a very convenient way to modify the cell genome at a specific locus. It involves transient transfection into mammalian cells of either (a) one or several plasmids coding for Cas9, the specific gRNA and eventually the sequence to be inserted, or (b) a mix of one or two plasmids and an RNA molecule (the gRNA).
Available application note: CRISPR/Cas9-mediated gene disruption using jetPRIME®.
siRNA transfection
jetPRIME® leads to over 90% knockdown of endogenous gene expression in a variety of cell lines. For example, jetPRIME®-mediated transfection of HeLa cells with 10 nM siRNA duplexes targeting endogenous lamin A/C in HeLa cells drastically reduces lamin A/C gene expression to barely detectable level (Fig. 1).
Fig. 1: Endogenous lamin A/C silencing using jetPRIME®. HeLa cells were transfected with 10 nM of 21-mer lamin A/C siRNA. After 48 h, lamin A/C silencing was assessed by immunofluorescence microscopy using an antibody against lamin A/C .
Co-transfection of different nucleic acids
jetPRIME® is well suited for DNA and siRNA/ miRNA co-transfection experiments or co-delivery of several DNA plasmids.
We performed DNA and siRNA delivery with jetPRIME® and observed highly efficient gene silencing in a variety of cell lines with very low toxicity. Over 90% silencing is achieved in adherent cells, using 10 nM siRNA (Fig. 2).
Fig. 2: Exogenous luciferase silencing in several cell lines after DNA & siRNA cotransfection using jetPRIME®. Experiments were performed with 400 ng p4CMV-Luc and 10 nM of luciferase siRNA per well in 6-well plates.
Quality
Every batch of jetPRIME® reagent is tested in-house by DNA transfection of HeLa cells with a GFP-expressing plasmid and each vial of reagent is provided with Certificate of Analysis.
Testimonials
Thank you PolyPlus for developing this reagent for researchers.
Protocol
To view our protocols, please fill in the fields below and click download.
Other files
Related blog posts
Bibliography
| Cell Line | in vitro in vivo | Delivered Molecule | Reagent | Results & Citations | |
|---|---|---|---|---|---|
| HEK-293T | in vitro | oligonucleotide and DNA cotransfection | jetPRIME | Frank, A. C. et al. (2019) Nat Commun 10, 1135 Apoptotic tumor cell-derived microRNA-375 uses CD36 to alter the tumor-associated macrophage phenotype | More details |
| U-2 OS | in vitro | DNA, siRNA | jetPRIME | McGarry, E. et al. (2016) Cancer Res 76, 2384-93 The Deubiquitinase USP9X Maintains DNA Replication Fork Stability and DNA Damage Checkpoint Responses by Regulating CLASPIN during S-Phase | More details |
| LLC-PK1 | in vitro | DNA, siRNA and DNA cotransfection | jetPRIME | Charbonney, E. et al. (2011) Mol Biol Cell 22, 4472-85 beta-catenin and Smad3 regulate the activity and stability of myocardin-related transcription factor during epithelial-myofibroblast transition | More details |
| Hep G2, Huh7 | in vitro | DNA, siRNA | jetPRIME | Han, M. et al. (2019) Biochem Biophys Res Commun 515, 455-461 XTP8 promotes hepatocellular carcinoma growth by forming a positive feedback loop with FOXM1 oncogene | More details |
| HeLa | in vitro | siRNA | jetPRIME | Perera, D. et al. (2016) Sci Rep 6, 29741 Oncogenic KRAS triggers MAPK-dependent errors in mitosis and MYC-dependent sensitivity to anti-mitotic agents | More details |
| HeLa | in vitro | DNA | jetPRIME | Chesnel, F. et al. (2015) Br J Cancer 113, 336-44 The von Hippel-Lindau tumour suppressor gene: uncovering the expression of the pVHL172 isoform | More details |
| THP-1 | in vitro | DNA | jetPRIME | Ishihara, Y. et al. (2019) Toxicol Sci , Interleukin 33 expression induced by aryl hydrocarbon receptor in macrophages | More details |
| MCF 10A | in vitro | DNA, siRNA | jetPRIME | Teo, C. R. et al. (2016) Cell Signal 28, 1479-88 The GNA13-RhoA signaling axis suppresses expression of tumor protective Kallikreins | More details |
| LX-2 | in vitro | mimic miRNA | jetPRIME | Coll, M. et al. (2015) Sci Rep 5, 11549 Integrative miRNA and Gene Expression Profiling Analysis of Human Quiescent Hepatic Stellate Cells | More details |
| A549 | in vitro | DNA | jetPRIME | Kruger, M. et al. (2019) J Oral Pathol Med , The impact of cigarette smoke on activity of single nucleotide polymorphisms of the vascular endothelial growth factor-promoter gene in cells of the upper aerodigestive tract | More details |