Overview

Specifications

Reagent

jetPRIME®

Molecule delivered

DNA, siRNA, DNA & siRNA

Applications

Plasmid transfection
Genome editing
Virus production
siRNA transfection
Co-delivery of different nucleic acids

Cell types

Adherent cell lines grown in presence of serum

Number of transfections

1.5 ml of jetPRIME® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates

Storage

5°C ± 3°C, stable for at least one year when stored appropriately

Provided with

jetPRIME buffer


Summary

jetPRIME® is a powerful and versatile DNA and siRNA transfection reagent for day-to-day experiments that leads to efficient and reliable scientific results. jetPRIME® ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. jetPRIME ® is also ideal for DNA/siRNA co-transfection or co-delivery of several plasmids. Furthermore, our jetPRIME® reagent is very gentle on cells since it requires low amounts of reagent and nucleic acid during transfection.

Ordering information

Reference NumberAmount of reagentAmount of buffer
114-010.1 ml5 ml
114-070.75 ml60 ml
114-151.5 ml2 x 60 ml
114-755 x 1.5 ml10 x 60 ml
712-60x60mL

1.5 ml is sufficient to perform ca.375 transfections in 6-well plates. Bulk quantities are available upon request. Please contact us.

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Description

Superior transfection efficiency

jetPRIME® is a powerful transfection reagent for day-to-day experiments. It leads to unusually high percentage of transfected adherent cell lines of various origins as well as primary cells.

Superior transfection efficiencies ranging between 70 and 90% were obtained with jetPRIME® reagent versus the top competitor’s reagent for several commonly used cell lines (Fig.1 & Fig.2).

jetPRIME - Versus Lipofectamine 2000

Fig. 1: Comparative transfection efficiency of jetPRIME versus its main competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection in 24-well plates. Conditions were used according to the manufacturer’s recommendation for both Lipofectamine® 2000 and for jetPRIME®.

jetPRIME - Versus Lipofectamine 3000

Fig. 2: Comparative transfection efficiency of jetPRIME® versus Lipofectamine® 3000. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection in 96-well plates or 24-well plates. Conditions were used according to the manufacturer’s recommendation for lipofectamine® 3000 and for jetPRIME®.

Cost-effective: less DNA and less reagent

jetPRIME® is a powerful in vitro transfection reagent that requires a small amount of reagent and plasmid DNA, making its use very cost-effective (Table 1).

jetPRIME - Recommended conditions

Table 1: Recommended conditions to use. Amounts of DNA and reagent (jetPRIME® and competitors) per well in 6-well plate for transfection according to manufacturers’ recommendations.

In addition to reducing costs, using less DNA also minimizes adverse cytotoxic effects triggered by transfection. Hence, jetPRIME® is the reagent of choice for high transfection efficiency with excellent cell viability.

Better cell viability

jetPRIME® is extremely gentle on cells during transfection leading to increased cell viability and improved transfection results. Cells transfected with jetPRIME® are healthy, while major cytotoxicity is observed with competitors (Fig.3).

jetPRIME - transfected cells compared to reagents L2K and L3K

Fig. 3: Cell viability 24h after transfection. Phase contrast microscopy of HeLa cells 24 h after transfections performed according to the manufacturer’s recommendations for each reagent.

Easy to use protocol

jetPRIME® is an easy-to-use transfection reagent (Fig.4):

  • Fast and easy to scale up and down
  • Compatible with serum and antibiotics

jetPRIME - protocol

Fig. 4: jetPRIME® protocol. Convenient protocol for DNA, siRNA and co-transfection of DNA and siRNA.

Take a look at our Expert Tips to ace DNA transfection in our blog section.

 

FAQ

If you have any questions regarding jetPRIME®, please visit our dedicated Frequently asked questions or contact us.

Applications

Plasmid transfection

jetPRIME® leads to remarkably high percentages of transfected adherent cell lines of various origin, as well as primary cells (Table 1).

jetPRIME - Plasmid transfection

Table 1: Transfection efficiency of various cell types using jetPRIME®. The percentage of GFP-positive cells was determined by FACS analysis 24 h after transfection.

Plasmids are small circular DNA molecules that are commonly found in bacteria. Plasmids exist and replicate separately from chromosomal DNA and in bacteria they often carry genes that are beneficial for bacterial survival. Plasmids can be deliberately introduced into desired cells and utilized to overexpress a gene of interest in a specific cell line. This procedure is called DNA transfection and is a commonly used method for studying gene function or protein of interest.

Read more…

Genome editing

The use of the CRISPR/Cas9 system in mammalian cells has recently emerged as a very convenient way to modify the cell genome at a specific locus. It involves transient transfection into mammalian cells of either (a) one or several plasmids coding for Cas9, the specific gRNA and eventually the sequence to be inserted, or (b) a mix of one or two plasmids and an RNA molecule (the gRNA).

Available application note: CRISPR/Cas9-mediated gene disruption using jetPRIME®.

Read more…

siRNA transfection

jetPRIME® leads to over 90% knockdown of endogenous gene expression in a variety of cell lines. For example, jetPRIME®-mediated transfection of HeLa cells with 10 nM siRNA duplexes targeting endogenous lamin A/C in HeLa cells drastically reduces lamin A/C gene expression to barely detectable level (Fig. 1).

jetPRIME - siRNA

Fig. 1: Endogenous lamin A/C silencing using jetPRIME®. HeLa cells were transfected with 10 nM of 21-mer lamin A/C siRNA. After 48 h, lamin A/C silencing was assessed by immunofluorescence microscopy using an antibody against lamin A/C .

Read more…

Co-transfection of different nucleic acids

jetPRIME® is well suited for DNA and siRNA/ miRNA co-transfection experiments or co-delivery of several DNA plasmids.

We performed DNA and siRNA delivery with jetPRIME® and observed highly efficient gene silencing in a variety of cell lines with very low toxicity. Over 90% silencing is achieved in adherent cells, using 10 nM siRNA (Fig. 2).

jetPRIME - cotransfection silencing

Fig. 2: Exogenous luciferase silencing in several cell lines after DNA & siRNA cotransfection using jetPRIME®. Experiments were performed with 400 ng p4CMV-Luc and 10 nM of luciferase siRNA per well in 6-well plates.

Read more…

Quality

Every batch of jetPRIME® reagent is tested in-house by DNA transfection of HeLa cells with a GFP-expressing plasmid and each vial of reagent is provided with Certificate of Analysis.

Testimonials

jetPRIME gave better transfection efficiencies than other reagents tested and much less cell death was noticed.  The cells don't seem to mind being treated with the transfection reagent, there is no need to change the media anyway.  I've already ordered a bigger volume of jetPRIME and have used half of it already! Will possibly be ordering some more soon!
 Kimberley S. 
 Royal Veterinary College, UK 
I already ordered jetPRIME. It was very efficient and easy to use! Also the cells were in a good state after transfection. Good product!
 Cosima R. 
 University Hospital Erlangen, DE  
Very good transfection efficiency, better value and ease of use
 Sean H. 
 MD Anderson Cancer Center, USA 
jetPRIME has low toxicity and with less amount of DNA and the transfection reagent comparable expression can be achieved. I would prefer using jetPRIME over other reagents for the above mentioned reasons.
 Vijayalakshmi A. 
 Baylor College of Medicine (BCM), USA 
Switching to jetPrime significantly impacted the success of our studies. In comparison to a competitors transfection reagent, jetPrime gives higher transfection efficiency, less toxicity and requires less plasmid DNA.
Thank you PolyPlus for developing this reagent for researchers.
 Marlene J. 
 Temple University, USA 
We're still in love with the jetPRIME transfection reagent. It was really the only reagent that was satisfactory for our immortalized primary MEFs and allowed two projects in our lab to move forward much more quickly than our originally planned lentiviral strategy. Bravo!





 Jeetayu B. 
 Albert Einstein College of Medicine, USA 
I was so pleasantly surprised by the efficiency of jetPRIME reagent I ordered some the next day. MCF7 cells are notoriously hard to transfect and they are our primary cell line in the lab so I am so thankful to have this reagent at a reasonable cost.
 Corinne H. 
 Ohio State University, USA 
Many thanks for the jetPRIME sample I have received, I am more than happy and appreciate your outstanding service. I recommended the reagent to many researchers and students in the department they will order some very soon.
 Esra A. 
 University of Sheffield, UK 
I'm very satisfied with jetPRIME. Good co-transfection efficiency with very low toxicity. One of the best I have ever tried.
 Diogo N. 
 CNRS Bordeaux, France 

Protocol

To view our protocols, please fill in the fields below and click download.

Other files

Bibliography

Order by :  
Found 1631 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
3T3-L1in vitroDNAjetPRIME
20-40% transfection efficiency
Standard protocol
A549, Epithelialin vitroDNAjetPRIME
70- 90% transfection efficiency
Standard protocol
A549, Epithelialin vitrosiRNA and DNA cotransfectionjetPRIME
90% silencing efficiency
Standard protocol
A549, Epithelialin vitrosiRNAjetPRIME
90-95% transfection efficiency at 10 nM siRNA
Protocol available
B16-F10, Epithelialin vitroDNAjetPRIME
70-80% transfection efficiency
Standard protocol
BNL CL.2, Epithelialin vitroDNAjetPRIME
50-60% transfection efficiency
Protocol available
BxPC-3, Epithelialin vitroDNAjetPRIME
Very good transfection efficiency
Standard protocol
C2C12, Myoblastin vitroDNAjetPRIME
70-90% transfection efficiency
Protocol available
C2C12, Myoblastin vitrosiRNAjetPRIME
80 % silencing efficiency at 50 nM siRNA
Protocol available
C3H/10T1/2, Fibroblastin vitroDNAjetPRIME
50-70 % transfection efficiency
Protocol available