Adherent and suspension cells
|Number of transfections|
1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates.
5°C ± 3°C, stable for 6 months (101-01N) to at least 12 months (other packaging sizes) when stored appropriately
101-01N, 101-10N, 101-40N and 101B-010N are provided with 150 mM NaCl solution for complex formation
jetPEI® transfection reagent is a linear polyethylenimine derivative, free of components of animal origin, providing highly effective and reproducible gene delivery to adherent and suspension cells. jetPEI® transfection reagent is therefore particularly well suited for automated or manual HTS (High Throughput Screening) with three protocols available: reverse, batch and forward.
|Reference Number||Amount of reagent||Amount of NaCl|
|101-10N||1 ml||50 ml|
|101-40N||4 x 1 ml||4 x 50 ml|
3 protocols to suit your application
In the forward protocol, the cells are split the day before transfection and the jetPEI®/DNA complexes are added to the adherent or suspension cells.
The reverse protocol is the most appropriate when transfecting a pool of genes, such as a DNA library (Fig. 1). In this protocol, the jetPEI®/DNA complexes are prepared or deposited in the wells prior to addition of the cells. Complexes are stable for up to 4 hours (Fig. 2).
The batch protocol has been developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is preferred for drug screening applications and allows rapid processing, one day faster than the forward protocol.
Fig. 1: jetPEI® reverse transfection protocol for HTS application.
Robust transfection complexes
Complexes formed with the water-soluble polymer jetPEI® and DNA allow efficient transfection for up to 4 hours, in contrast to lipid-based reagents and calcium phosphate. Thus they allow plenty of time to dispense the complexes into the plates (Fig. 2).
Fig. 2: Effect of complex formation incubation time on transfection efficiency with jetPEI®. HEK-293 cells were transfected in 96-well plates with pCMVLuc and jetPEI® following the reverse transfection protocol. Luciferase activity was measured after 24 h.
Batch to batch reproducibility
HTS DNA transfection using jetPEI® gives highly consistent transfection efficiency from batch-to-batch (Fig. 3).
Fig. 3: Batch-to-batch reproducibility using jetPEI®. For each lot, HeLa cells were transfected in triplicate in the presence of serum using the standard protocol for a 24-well plate.
Efficient in a wide range of cell types
jetPEI® successfully delivers genes to various adherent and non-adherent cell lines, as well as primary cells (Table 1). Over 550 publications using jetPEI® can be found in the Polyplus-transfection Database. In addition, our online Database gives specific transfection conditions for over 400 cell lines and primary cells.
Table 1: Some common cell lines and primary cells successfully transfected using jetPEI®.
Superior transfection results
jetPEI® was compared to several other popular transfection reagents (Fig. 4). jetPEI® was found to offer the best performance: high efficiency and low variability (small standard deviation).
Fig. 4: Transfection efficiency of a series of commercial reagents. HeLa cells were transfected in 24-well plates in the presence of 10% serum, using 1 µg pCMV-luciferase according to the manufacturers’ protocols. Luciferase expression was measured 24 h after transfection.
Our jetPEI® reagent is perfectly well suited for plasmid DNA transfection, especially for High-Troughput Screening (HTS) application.
Every batch of jetPEI® is tested in-house by DNA transfection of HeLa cells. Transfection with a firefly Luciferase gene under the control of CMV promoter gives at least 109 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.
To view our protocols, please fill in the fields below and click download.
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