Overview

Specifications

Reagent

jetPEI®

Molecule delivered

DNA

Applications

Plasmid transfection
High Throughput Screening (HTS)

Cell types

Adherent and suspension cells

Number of transfections

1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates.

Storage

Store jetPEI® at 5 °C ± 3°C.
Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.

Provided with

101-01N, 101-10N, 101-40N and 101B-010N are provided with 150 mM NaCl solution for complex formation


Summary

jetPEI® transfection reagent is a linear polyethylenimine derivative, free of components of animal origin, providing highly effective and reproducible gene delivery to adherent and suspension cells. jetPEI® transfection reagent is therefore particularly well suited for automated or manual HTS (High Throughput Screening) with three protocols available: reverse, batch and forward.

Ordering information

Reference NumberAmount of reagentAmount of NaCl
101000053jetPEI® 1 mL50 ml
101000020jetPEI® 4 x 1 mL4 x 50 ml

Description

3 protocols to suit your application

In the forward protocol, the cells are split the day before transfection and the jetPEI®/DNA complexes are added to the adherent or suspension cells.

The reverse protocol is the most appropriate when transfecting a pool of genes, such as a DNA library (Fig. 1). In this protocol, the jetPEI®/DNA complexes are prepared or deposited in the wells prior to addition of the cells. Complexes are stable for up to 4 hours (Fig. 2).

The batch protocol has been developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is preferred for drug screening applications and allows rapid processing, one day faster than the forward protocol.

jetPEI - HTS Protocol

Fig. 1: jetPEI® reverse transfection protocol for HTS application.

Robust transfection complexes

Complexes formed with the water-soluble polymer jetPEI® and DNA allow efficient transfection for up to 4 hours, in contrast to lipid-based reagents and calcium phosphate. Thus they allow plenty of time to dispense the complexes into the plates (Fig. 2).

jetPEI - Complexe stability

Fig. 2: Effect of complex formation incubation time on transfection efficiency with jetPEI®. HEK-293 cells were transfected in 96-well plates with pCMVLuc and jetPEI® following the reverse transfection protocol. Luciferase activity was measured after 24 h.

Batch to batch reproducibility

HTS DNA transfection using jetPEI® gives highly consistent transfection efficiency from batch-to-batch (Fig. 3).

jetPEI - Comparison batchs

Fig. 3: Batch-to-batch reproducibility using jetPEI®. For each lot, HeLa cells were transfected in triplicate in the presence of serum using the standard protocol for a 24-well plate.

Efficient in a wide range of cell types

jetPEI® successfully delivers genes to various adherent and non-adherent cell lines, as well as primary cells (Table 1). Over 550 publications using jetPEI® can be found in the Polyplus-transfection Database. In addition, our online Database gives specific transfection conditions for over 400 cell lines and primary cells.

jetPEI - Examples of successfully transfected cells lines (without title)

Table 1: Some common cell lines and primary cells successfully transfected using jetPEI®.

Superior transfection results

jetPEI® was compared to several other popular transfection reagents (Fig. 4). jetPEI® was found to offer the best performance: high efficiency and low variability (small standard deviation).

jetPEI - Transfection results

Fig. 4: Transfection efficiency of a series of commercial reagents. HeLa cells were transfected in 24-well plates in the presence of 10% serum, using 1 µg pCMV-luciferase according to the manufacturers’ protocols. Luciferase expression was measured 24 h after transfection.

FAQ

If you have any questions regarding jetPEI®, please visit our dedicated Frequently asked questions or contact us.

Applications

Our jetPEI® reagent is perfectly well suited for plasmid DNA transfection, especially for High-Troughput Screening (HTS) application.

Read more…

Quality

Every batch of jetPEI® is tested in-house  by DNA transfection of HeLa cells. Transfection with a firefly Luciferase gene under the control of CMV promoter gives at least 109 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.

Protocol

In order to download a product protocol or a certificate of analysis, please create an account on Polyplus-transfection® Portal.

Why would you need to create an account?

In this personal area you will have access to:

  • Product Protocols
  • Certificates of Analysis
  • Exclusive webinars/articles
  • And surprise features!

Create or Access your account

Other files

Related blog posts

Bibliography

Order by :  
Found 1831 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
HeLa, Macrophagesin vitroDNAjetPEI, jetPRIME
Guennewig, B. et al. (2014)

RNA 20, 61-75
Synthetic pre-microRNAs reveal dual-strand activity of miR-34a on TNF-alpha
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MIN6in vitroDNAjetPEI, jetPRIME
Hofmeister-Brix, A. et al. (2013)

J Biol Chem 288, 35824-39
Identification of the ubiquitin-like domain of midnolin as a new glucokinase interaction partner
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HeLain vitroDNAjetPEI
Ingle, N. P. et al. (2013)

Mol Pharm 10, 4120-35
Spatiotemporal cellular imaging of polymer-pDNA nanocomplexes affords in situ morphology and trafficking trends
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CHO-K1in vitroDNAjetPEI
Kern, F. et al. (2013)

Biochem J 455, 217-27
Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress
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HeLa Kyotoin vitroDNAjetPEI
Kress, E. et al. (2013)

J Cell Biol 201, 559-75
The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A
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JB6, NIH/3T3in vitroDNAjetPEI
Lee, C. J. et al. (2014)

Carcinogenesis 35, 432-41
Targeting of magnolin on ERKs inhibits Ras/ERKs/RSK2-signaling-mediated neoplastic cell transformation
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HeLain vitromRNAjetPEI
Bire, S. et al. (2013)

BMC Biotechnol ,
Exogenous mRNA delivery and bioavailability in gene transfer mediated by piggyBac transposition.
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CNE-1in vitroDNAjetPEI
Li, B. et al. (2013)

BMC Cancer ,
Increased phosphorylation of histone H3 at serine 10 is involved in Epstein-Barr virus latent membrane protein-1-induced carcinogenesis of nasopharyngeal carcinoma
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Flp-In-293, U-2 OSin vitroDNA, siRNAjetPEI, jetPRIME
Wu, K. Z. et al. (2016)

Nucleic Acids Res 44, 8786-8798
DDK dependent regulation of TOP2A at centromeres revealed by a chemical genetics approach
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SW480in vitroDNAjetPEI
Maamer-Azzabi, A. et al. (2013)

Cell Death Dis 4, e801
Metastatic SW620 colon cancer cells are primed for death when detached and can be sensitized to anoikis by the BH3-mimetic ABT-737
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