Overview

Specifications

Reagent

jetPEI®

Molecule delivered

DNA

Applications

Plasmid transfection
High Throughput Screening (HTS)

Cell types

Adherent and suspension cells

Number of transfections

1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates.

Storage

Store jetPEI® at 5 °C ± 3°C.
Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.

Provided with

101-01N, 101-10N, 101-40N and 101B-010N are provided with 150 mM NaCl solution for complex formation


Summary

jetPEI® transfection reagent is a linear polyethylenimine derivative, free of components of animal origin, providing highly effective and reproducible gene delivery to adherent and suspension cells. jetPEI® transfection reagent is therefore particularly well suited for automated or manual HTS (High Throughput Screening) with three protocols available: reverse, batch and forward.

Ordering information

Reference NumberAmount of reagentAmount of NaCl
101000053jetPEI® 1 mL50 ml
101000020jetPEI® 4 x 1 mL4 x 50 ml

Description

3 protocols to suit your application

In the forward protocol, the cells are split the day before transfection and the jetPEI®/DNA complexes are added to the adherent or suspension cells.

The reverse protocol is the most appropriate when transfecting a pool of genes, such as a DNA library (Fig. 1). In this protocol, the jetPEI®/DNA complexes are prepared or deposited in the wells prior to addition of the cells. Complexes are stable for up to 4 hours (Fig. 2).

The batch protocol has been developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is preferred for drug screening applications and allows rapid processing, one day faster than the forward protocol.

jetPEI - HTS Protocol

Fig. 1: jetPEI® reverse transfection protocol for HTS application.

Robust transfection complexes

Complexes formed with the water-soluble polymer jetPEI® and DNA allow efficient transfection for up to 4 hours, in contrast to lipid-based reagents and calcium phosphate. Thus they allow plenty of time to dispense the complexes into the plates (Fig. 2).

jetPEI - Complexe stability

Fig. 2: Effect of complex formation incubation time on transfection efficiency with jetPEI®. HEK-293 cells were transfected in 96-well plates with pCMVLuc and jetPEI® following the reverse transfection protocol. Luciferase activity was measured after 24 h.

Batch to batch reproducibility

HTS DNA transfection using jetPEI® gives highly consistent transfection efficiency from batch-to-batch (Fig. 3).

jetPEI - Comparison batchs

Fig. 3: Batch-to-batch reproducibility using jetPEI®. For each lot, HeLa cells were transfected in triplicate in the presence of serum using the standard protocol for a 24-well plate.

Efficient in a wide range of cell types

jetPEI® successfully delivers genes to various adherent and non-adherent cell lines, as well as primary cells (Table 1). Over 550 publications using jetPEI® can be found in the Polyplus-transfection Database. In addition, our online Database gives specific transfection conditions for over 400 cell lines and primary cells.

jetPEI - Examples of successfully transfected cells lines (without title)

Table 1: Some common cell lines and primary cells successfully transfected using jetPEI®.

Superior transfection results

jetPEI® was compared to several other popular transfection reagents (Fig. 4). jetPEI® was found to offer the best performance: high efficiency and low variability (small standard deviation).

jetPEI - Transfection results

Fig. 4: Transfection efficiency of a series of commercial reagents. HeLa cells were transfected in 24-well plates in the presence of 10% serum, using 1 µg pCMV-luciferase according to the manufacturers’ protocols. Luciferase expression was measured 24 h after transfection.

FAQ

If you have any questions regarding jetPEI®, please visit our dedicated Frequently asked questions or contact us.

Applications

Our jetPEI® reagent is perfectly well suited for plasmid DNA transfection, especially for High-Troughput Screening (HTS) application.

Read more…

Quality

Every batch of jetPEI® is tested in-house  by DNA transfection of HeLa cells. Transfection with a firefly Luciferase gene under the control of CMV promoter gives at least 109 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.

Protocol

In order to download a product protocol or a certificate of analysis, please create an account on Polyplus® Portal.

Why would you need to create an account?

In this personal area you will have access to:

  • Product Protocols
  • Certificates of Analysis
  • Exclusive webinars/articles
  • And surprise features!

Create or Access your account

Other files

Related blog posts

Bibliography

Order by :  
Found 1837 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
CHO, HEK-293in vitroDNAjetPEI
Dubel, S. J. et al. (2004)

J Biol Chem 279, 29263-9
Plasma membrane expression of T-type calcium channel alpha(1) subunits is modulated by high voltage-activated auxiliary subunits
More details
HEK-293Tin vitroDNAjetPEI
Benleulmi-Chaachoua, A. et al. (2018)

Cell Mol Life Sci ,
Melatonin receptors limit dopamine reuptake by regulating dopamine transporter cell-surface exposure
More details
HeLain vitroDNAjetPEI
Leyva-Illades, D. et al. (2014)

J Neurosci 34, 14079-95
SLC30A10 is a cell surface-localized manganese efflux transporter, and parkinsonism-causing mutations block its intracellular trafficking and efflux activity
More details
MCF7, MDA-MB-231in vitroDNAjetPEI
Ennen, M. et al. (2011)

Free Radic Biol Med 50, 1771-9
Regulation of the high basal expression of the manganese superoxide dismutase gene in aggressive breast cancer cells
More details
HEK-293Tin vitroDNAjetPEI
Cho, J. H. et al. (2014)

Eur J Pharmacol 741, 247-53
Sulforaphane inhibition of TPA-mediated PDCD4 downregulation contributes to suppression of c-Jun and induction of p21-dependent Nrf2 expression
More details
BGC-803in vitroDNAjetPEI
Liu, Z. et al. (2014)

Mol Med Rep 10, 2459-64
Calcium/calmodulindependent protein kinase II enhances metastasis of human gastric cancer by upregulating nuclear factorkappaB and Aktmediated matrix metalloproteinase9 production
More details
MLE 12in vitroDNAjetPEI
Farrell, M. R. et al. (2010)

Free Radic Biol Med 49, 1361-7
Thioredoxin-interacting protein inhibits hypoxia-inducible factor transcriptional activity
More details
HEK-293Tin vitroDNAjetPEI
Habibian, M. et al. (2018)

Nucleic Acids Res 46, 1614-1623
Structural properties and gene-silencing activity of chemically modified DNA-RNA hybrids with parallel orientation
More details
GC.92, RG37in vitroDNAjetPEI
Mamouni, K. et al. (2014)

Mol Cell Biol 34, 3144-55
RhoB promotes gammaH2AX dephosphorylation and DNA double-strand break repair
More details
Mouse primary hepatocytesin vitroDNAjetPEI
Fullerton, M. D. et al. (2010)

Metabolism 59, 1691-700
Complementation of the metabolic defect in CTP:phosphoethanolamine cytidylyltransferase (Pcyt2)-deficient primary hepatocytes
More details