Overview

Specifications

Reagent

jetPEI®

Molecule delivered

DNA

Applications

Plasmid transfection
High Throughput Screening (HTS)

Cell types

Adherent and suspension cells

Number of transfections

1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates.

Storage

5°C ± 3°C, stable for at least one year when stored appropriately

Provided with

101-01N, 101-10N, 101-40N and 101B-010N are provided with 150 mM NaCl solution for complex formation


Summary

jetPEI® transfection reagent is a linear polyethylenimine derivative, free of components of animal origin, providing highly effective and reproducible gene delivery to adherent and suspension cells. jetPEI® transfection reagent is therefore particularly well suited for automated or manual HTS (High Throughput Screening) with three protocols available: reverse, batch and forward.

Ordering information

Reference NumberAmount of reagentAmount of NaCl
101-10N1 ml50 ml
101-40N4 x 1 ml4 x 50 ml

Description

3 protocols to suit your application

In the forward protocol, the cells are split the day before transfection and the jetPEI®/DNA complexes are added to the adherent or suspension cells.

The reverse protocol is the most appropriate when transfecting a pool of genes, such as a DNA library (Fig. 1). In this protocol, the jetPEI®/DNA complexes are prepared or deposited in the wells prior to addition of the cells. Complexes are stable for up to 4 hours (Fig. 2).

The batch protocol has been developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is preferred for drug screening applications and allows rapid processing, one day faster than the forward protocol.

jetPEI - HTS Protocol

Fig. 1: jetPEI® reverse transfection protocol for HTS application.

Robust transfection complexes

Complexes formed with the water-soluble polymer jetPEI® and DNA allow efficient transfection for up to 4 hours, in contrast to lipid-based reagents and calcium phosphate. Thus they allow plenty of time to dispense the complexes into the plates (Fig. 2).

jetPEI - Complexe stability

Fig. 2: Effect of complex formation incubation time on transfection efficiency with jetPEI®. HEK-293 cells were transfected in 96-well plates with pCMVLuc and jetPEI® following the reverse transfection protocol. Luciferase activity was measured after 24 h.

Batch to batch reproducibility

HTS DNA transfection using jetPEI® gives highly consistent transfection efficiency from batch-to-batch (Fig. 3).

jetPEI - Comparison batchs

Fig. 3: Batch-to-batch reproducibility using jetPEI®. For each lot, HeLa cells were transfected in triplicate in the presence of serum using the standard protocol for a 24-well plate.

Efficient in a wide range of cell types

jetPEI® successfully delivers genes to various adherent and non-adherent cell lines, as well as primary cells (Table 1). Over 550 publications using jetPEI® can be found in the Polyplus-transfection Database. In addition, our online Database gives specific transfection conditions for over 400 cell lines and primary cells.

jetPEI - Examples of successfully transfected cells lines (without title)

Table 1: Some common cell lines and primary cells successfully transfected using jetPEI®.

Superior transfection results

jetPEI® was compared to several other popular transfection reagents (Fig. 4). jetPEI® was found to offer the best performance: high efficiency and low variability (small standard deviation).

jetPEI - Transfection results

Fig. 4: Transfection efficiency of a series of commercial reagents. HeLa cells were transfected in 24-well plates in the presence of 10% serum, using 1 µg pCMV-luciferase according to the manufacturers’ protocols. Luciferase expression was measured 24 h after transfection.

FAQ

If you have any questions regarding jetPEI®, please visit our dedicated Frequently asked questions or contact us.

Applications

Our jetPEI® reagent is perfectly well suited for plasmid DNA transfection, especially for High-Troughput Screening (HTS) application.

Read more…

Quality

Every batch of jetPEI® is tested in-house  by DNA transfection of HeLa cells. Transfection with a firefly Luciferase gene under the control of CMV promoter gives at least 109 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.

Protocol

In order to download a product protocol or a certificate of analysis, please create an account on Polyplus-transfection® Portal.

Why would you need to create an account?

In this personal area you will have access to:

  • Product Protocols
  • Certificates of Analysis
  • Exclusive webinars/articles
  • And surprise features!

Create or Access your account

Other files

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Bibliography

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Frauenstein, K. et al. (2013)

Cell Death Differ 20, 1425-34
Evidence for a novel anti-apoptotic pathway in human keratinocytes involving the aryl hydrocarbon receptor, E2F1, and checkpoint kinase 1
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Granato, M. et al. (2013)

PLoS One 8, e75965
JNK and macroautophagy activation by bortezomib has a pro-survival effect in primary effusion lymphoma cells
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Havermann, S. et al. (2013)

J Agric Food Chem 61, 2158-64
Molecular effects of baicalein in Hct116 cells and Caenorhabditis elegans : activation of the Nrf2 signaling pathway and prolongation of lifespan
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Huang, A. F. et al. (2013)

Mol Cancer ,
CD164 regulates the tumorigenesis of ovarian surface epithelial cells through the SDF-1
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HEK-293Tin vitroDNAjetPEI
Kang, K. et al. (2013)

J Biol Chem 288, 25414-27
MicroRNA-124 suppresses the transactivation of nuclear factor of activated T cells by targeting multiple genes and inhibits the proliferation of pulmonary artery smooth muscle cells
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NCI-H1650, HEK-293T, NIH/3T3in vitroDNAjetPEI
Kim, M. O. et al. (2014)

Carcinogenesis 35, 683-91
[6]-shogaol inhibits growth and induces apoptosis of non-small cell lung cancer cells by directly regulating Akt1/2
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HeLa, Rat embryonic fibroblastsin vitroDNAjetPEI
Lavelin, I. et al. (2013)

PLoS One 8, e73549
Differential effect of actomyosin relaxation on the dynamic properties of focal adhesion proteins
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HCT 116, SW480in vitromimic miRNA, mimic miRNA and DNA cotransfection, siRNAINTERFERin, jetPEI
Wang, Z. et al. (2015)

Cancer Cell Int 15, 40
MicroRNA-378-5p suppresses cell proliferation and induces apoptosis in colorectal cancer cells by targeting BRAF
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Lee, S. Y. et al. (2013)

Cancer Prev Res (Phila) 6, 1356-64
Esculetin suppresses proliferation of human colon cancer cells by directly targeting beta-catenin
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Human SV-40 immortalized fibroblastsin vitroDNA, siRNAINTERFERin, jetPEI
Guirouilh-Barbat, J. et al. (2016)

PLoS Genet 12, e1006230
53BP1 Protects against CtIP-Dependent Capture of Ectopic Chromosomal Sequences at the Junction of Distant Double-Strand Breaks
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