Overview

Specifications

Reagent

jetPEI®

Molecule delivered

DNA

Applications

Plasmid transfection
High Throughput Screening (HTS)

Cell types

Adherent and suspension cells

Number of transfections

1 ml of jetPEI® is sufficient to perform up to 2000 transfections in 96-well plates.

Storage

5°C ± 3°C, stable for 6 months (101-01N) to at least 12 months (other packaging sizes) when stored appropriately

Provided with

101-01N, 101-10N, 101-40N and 101B-010N are provided with 150 mM NaCl solution for complex formation


Summary

jetPEI® transfection reagent is a linear polyethylenimine derivative, free of components of animal origin, providing highly effective and reproducible gene delivery to adherent and suspension cells. jetPEI® transfection reagent is therefore particularly well suited for automated or manual HTS (High Throughput Screening) with three protocols available: reverse, batch and forward.

Ordering information

Reference NumberAmount of reagentAmount of NaCl
101-10N1 ml50 ml
101-40N4 x 1 ml4 x 50 ml

Description

3 protocols to suit your application

In the forward protocol, the cells are split the day before transfection and the jetPEI®/DNA complexes are added to the adherent or suspension cells.

The reverse protocol is the most appropriate when transfecting a pool of genes, such as a DNA library (Fig. 1). In this protocol, the jetPEI®/DNA complexes are prepared or deposited in the wells prior to addition of the cells. Complexes are stable for up to 4 hours (Fig. 2).

The batch protocol has been developed to prepare a homogeneous pool of transfected cells. For this purpose, the cells are transfected just after trypsinization, while still in suspension. This protocol is preferred for drug screening applications and allows rapid processing, one day faster than the forward protocol.

jetPEI - HTS Protocol

Fig. 1: jetPEI® reverse transfection protocol for HTS application.

Robust transfection complexes

Complexes formed with the water-soluble polymer jetPEI® and DNA allow efficient transfection for up to 4 hours, in contrast to lipid-based reagents and calcium phosphate. Thus they allow plenty of time to dispense the complexes into the plates (Fig. 2).

jetPEI - Complexe stability

Fig. 2: Effect of complex formation incubation time on transfection efficiency with jetPEI®. HEK-293 cells were transfected in 96-well plates with pCMVLuc and jetPEI® following the reverse transfection protocol. Luciferase activity was measured after 24 h.

Batch to batch reproducibility

HTS DNA transfection using jetPEI® gives highly consistent transfection efficiency from batch-to-batch (Fig. 3).

jetPEI - Comparison batchs

Fig. 3: Batch-to-batch reproducibility using jetPEI®. For each lot, HeLa cells were transfected in triplicate in the presence of serum using the standard protocol for a 24-well plate.

Efficient in a wide range of cell types

jetPEI® successfully delivers genes to various adherent and non-adherent cell lines, as well as primary cells (Table 1). Over 550 publications using jetPEI® can be found in the Polyplus-transfection Database. In addition, our online Database gives specific transfection conditions for over 400 cell lines and primary cells.

jetPEI - Examples of successfully transfected cells lines (without title)

Table 1: Some common cell lines and primary cells successfully transfected using jetPEI®.

Superior transfection results

jetPEI® was compared to several other popular transfection reagents (Fig. 4). jetPEI® was found to offer the best performance: high efficiency and low variability (small standard deviation).

jetPEI - Transfection results

Fig. 4: Transfection efficiency of a series of commercial reagents. HeLa cells were transfected in 24-well plates in the presence of 10% serum, using 1 µg pCMV-luciferase according to the manufacturers’ protocols. Luciferase expression was measured 24 h after transfection.

FAQ

If you have any questions regarding jetPEI®, please visit our dedicated Frequently asked questions or contact us.

Applications

Our jetPEI® reagent is perfectly well suited for plasmid DNA transfection, especially for High-Troughput Screening (HTS) application.

Read more…

Quality

Every batch of jetPEI® is tested in-house  by DNA transfection of HeLa cells. Transfection with a firefly Luciferase gene under the control of CMV promoter gives at least 109 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.

Protocol

To view our protocols, please fill in the fields below and click download.

Other files

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Bibliography

Order by :  
Found 1815 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
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Linnewiel, K. et al. (2009)

Free Radic Biol Med 47, 659-67
Structure activity relationship of carotenoid derivatives in activation of the electrophile/antioxidant response element transcription system
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Sun, C. et al. (2014)

PLoS One 9, e108839
Elevation of proteasomal substrate levels sensitizes cells to apoptosis induced by inhibition of proteasomal deubiquitinases
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Chen, L. H. et al. (2012)

Int J Mol Sci 13, 1209-24
Targeting protective autophagy exacerbates UV-triggered apoptotic cell death
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HEK-293in vitroDNAjetPEI
Loewith, R. et al. (2002)

Mol Cell 10, 457-68
Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control
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Wang, W. M. et al. (2014)

Br J Dermatol 171, 356-62
Biochemical properties of the recurrent LMX1b truncated mutant carried in a Taiwanese family with nail-patella syndrome
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Cremasco, V. et al. (2012)

J Bone Miner Res 27, 2452-63
Protein kinase C-delta deficiency perturbs bone homeostasis by selective uncoupling of cathepsin K secretion and ruffled border formation in osteoclasts
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Maguire, K. et al. (2009)

BMC Mol Biol 10, 15
Genetic correction of splice site mutation in purified and enriched myoblasts isolated from mdx5cv mice
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Huh-7.5.1in vitroshRNA plasmidjetPEI
Zhao, Y. et al. (2014)

J Immunol 193, 783-96
Ficolin-2 inhibits hepatitis C virus infection, whereas apolipoprotein E3 mediates viral immune escape
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RKOin vitroDNAjetPEI
Demidov, O. N. et al. (2012)

Cell Death Differ 19, 1761-8
Role of Gadd45a in Wip1-dependent regulation of intestinal tumorigenesis
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HeLain vitroDNA, siRNAINTERFERin, jetPEI
Massip, A. et al. (2013)

FEBS Lett 587, 3188-94
E2F1 activates p53 transcription through its distal site and participates in apoptosis induction in HPV-positive cells
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