Overview

Specifications

Reagent

jetPEI®-Macrophage

Molecule delivered

DNA

Applications

DNA transfection of primary macrophages, glial & dendritic cells

Cell types

Primary macrophages, glial & dendritic cells

Number of transfections

0.5 ml of jetPEI®-Macrophage transfection reagent is sufficient to perform up to 500 transfections in 24-well plates or 160 transfections in 60-mm dishes

Storage

5°C ± 3°C, stable for at least one year when stored appropriately

Provided with

150 mM NaCl


Summary

jetPEI®-Macrophage transfects primary macrophages, glial & dendritic cells that are considered difficult to transfect. It contains a mannose-conjugated linear polyethylenimine that binds to cells expressing mannose-specific membrane receptors, such as macrophages.

jetPEI®-Macrophage is also the reagent of choice for the transfection of cell lines such as RAW 264.7.

jetPEI®-Macrophage is a Mannose-bearing linear polyethylenimine designed to enhance the transfection of cells expressing Mannose receptors on their membrane. Cell targeting is the result of binding of the Mannose residues to the specific cell-surface receptors, leading to internalization of the DNA complexes.

Ordering information

Reference NumberAmount of reagentAmount of NaCl
103-05N0.5 ml50 ml

0.5 ml of jetPEI®-Macrophage transfection reagent is sufficient to perform up to 500 transfections in 24-well plates.

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Description

Transfection of primary human macrophages

jetPEI®-Macrophage allows successful transfection of macrophages derived from monocytes maturated for 7 days in presence of GM-CSF (Fig.1).

jet-PEI-Macrophage-fig1-vc

Fig. 1: Transfection of human macrophages with jetPEI®-Macrophage. Human macrophages expressing Beta-Galactosidase after transfection using jetPEI®-Macrophage.

Higher transfection efficiency compared to versatile jetPEI®

Primary human macrophages and murine RAW 264.7 cells express significantly higher protein levels when transfected with jetPEI®-Macrophage (Fig. 2).

jetPEI-Macrophage - comparison jetPEI

Fig. 2: Comparative efficiency of jetPEI®-Macrophage versus jetPEI® on primary human macrophages and murine RAW 264.7 cells in 24-well plates. Primary human macrophages were transfected in the presence of 100 U/ml GM-CSF and 10% serum, using 1 µg pCMVLuc and 2 µl transfection reagent. Murine RAW 264.7 cells were transfected using 2 µg pCMVLuc and 6.4 µl transfection reagent. Luciferase activity was measured 24 h post-transfection.

Easy to use protocol

The jetPEI®-Macrophage protocol is as simple as the jetPEI® one: Mix the DNA with the reagent to form complexes and simply add the mixture to the cells. jetPEI®-Macrophage is compatible with serum and antibiotics, thus eliminating the need for media change. Protein expression is determined 24 h to 72 h post-transfection.

Applications

jetPEI®-Macrophage is perfectly suited for plasmid delivery (DNA, shRNA or miRNA) to cells that express mannose-specific membrane receptors, such as primary macrophages, glial & dendritic cells.

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Quality

Every batch of jetPEI®-Macrophage is tested in a transfection assay. Typically, transfection of a firefly luciferase gene under the control of the CMV promote gives 109 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.

Protocol

In order to download a product protocol or a certificate of analysis, please create an account on Polyplus-transfection® Portal.

Why would you need to create an account?

In this personal area you will have access to:

  • Product Protocols
  • Certificates of Analysis
  • Exclusive webinars/articles
  • And surprise features!

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Other files

Bibliography

Order by :  
Found 72 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
Human macrophagesin vitroDNA, miRNA plasmidjetPEI-Macrophage
Fredman, G. et al. (2012)

Sci Rep 2, 639
Self-limited versus delayed resolution of acute inflammation: temporal regulation of pro-resolving mediators and microRNA
More details
THP-1in vitroDNAjetPEI-Macrophage
Iwamoto, N. et al. (2007)

Circ Res 101, 156-65
ATP-binding cassette transporter A1 gene transcription is downregulated by activator protein 2alpha. Doxazosin inhibits activator protein 2alpha and increases high-density lipoprotein biogenesis independent of alpha1-adrenoceptor blockade
More details
RAW 264.7in vitroDNAjetPEI-Macrophage
Quinn, S. R. et al. (2014)

J Biol Chem 289, 4316-25
The role of Ets2 transcription factor in the induction of microRNA-155 (miR-155) by lipopolysaccharide and its targeting by interleukin-10
More details
RAW 264.7in vitroDNAjetPEI-Macrophage
Xiang, Q. et al. (2009)

J Int Med Res 37, 491-502
Endotoxin tolerance of RAW264.7 correlates with p38-dependent up-regulation of scavenger receptor-A
More details
Mouse bone marrow-derived denditric cellsin vitroDNAjetPEI-Macrophage
Jannuzzi, G. P. et al. (2015)

PLoS One 10, e0129401
scFv from Antibody That Mimics gp43 Modulates the Cellular and Humoral Immune Responses during Experimental Paracoccidioidomycosis
More details
Mouse bone marrow-derived macrophagesin vitroDNAjetPEI-Macrophage
Chen, W. et al. (2018)

J Cell Physiol 233, 6975-6983
Mig6 reduces inflammatory mediators production by regulating the activation of EGFR in LPS-induced endotoxemia
More details
RAW 264.7in vitroDNA, shRNA plasmidjetPEI-Macrophage
Pei, G. et al. (2014)

J Cell Sci ,
Identification of an immune regulated phagosomal Rab cascade in macrophages
More details
Human monocyte-derived macrophagesin vitroDNAjetPEI-Macrophage
Ding, L. et al. (2017)

J Biol Chem ,
Akt3 Kinase Suppresses Pinocytosis of Low-Density-Lipoprotein By Macrophages via Novel WNK/SGK1/Cdc42 Pathway
More details
Human monocyte-derived macrophagesin vitroDNAjetPEI-Macrophage
Billiet, L. et al. (2005)

J Biol Chem 280, 40310-8
Extracellular human thioredoxin-1 inhibits lipopolysaccharide-induced interleukin-1beta expression in human monocyte-derived macrophages
More details
RAW 264.7in vitroDNAjetPEI-Macrophage
Guo, H. et al. (2010)

J Biol Chem 285, 37787-96
Osteopontin and protein kinase C regulate PDLIM2 activation and STAT1 ubiquitination in LPS-treated murine macrophages
More details