Molecule delivered



DNA transfection of primary macrophages, glial & dendritic cells

Cell types

Primary macrophages, glial & dendritic cells

Number of transfections

0.5 ml of jetPEI®-Macrophage transfection reagent is sufficient to perform up to 500 transfections in 24-well plates or 160 transfections in 60-mm dishes


5°C ± 3°C, for up to 12 months

Provided with

150 mM NaCl


jetPEI®-Macrophage transfects primary macrophages, glial & dendritic cells that are considered difficult to transfect. It contains a mannose-conjugated linear polyethylenimine that binds to cells expressing mannose-specific membrane receptors, such as macrophages.

jetPEI®-Macrophage is also the reagent of choice for the transfection of cell lines such as RAW 264.7.

jetPEI®-Macrophage is a Mannose-bearing linear polyethylenimine designed to enhance the transfection of cells expressing Mannose receptors on their membrane. Cell targeting is the result of binding of the Mannose residues to the specific cell-surface receptors, leading to internalization of the DNA complexes.

Ordering information

Reference NumberAmount of reagentAmount of NaCl
103-05N0.5 ml50 ml

0.5 ml of jetPEI®-Macrophage transfection reagent is sufficient to perform up to 500 transfections in 24-well plates.

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Transfection of primary human macrophages

jetPEI®-Macrophage allows successful transfection of macrophages derived from monocytes maturated for 7 days in presence of GM-CSF (Fig.1).


Fig. 1: Transfection of human macrophages with jetPEI®-Macrophage. Human macrophages expressing Beta-Galactosidase after transfection using jetPEI®-Macrophage.

Higher transfection efficiency compared to versatile jetPEI®

Primary human macrophages and murine RAW 264.7 cells express significantly higher protein levels when transfected with jetPEI®-Macrophage (Fig. 2).

jetPEI-Macrophage - comparison jetPEI

Fig. 2: Comparative efficiency of jetPEI®-Macrophage versus jetPEI® on primary human macrophages and murine RAW 264.7 cells in 24-well plates. Primary human macrophages were transfected in the presence of 100 U/ml GM-CSF and 10% serum, using 1 µg pCMVLuc and 2 µl transfection reagent. Murine RAW 264.7 cells were transfected using 2 µg pCMVLuc and 6.4 µl transfection reagent. Luciferase activity was measured 24 h post-transfection.

Easy to use protocol

The jetPEI®-Macrophage protocol is as simple as the jetPEI® one: Mix the DNA with the reagent to form complexes and simply add the mixture to the cells. jetPEI®-Macrophage is compatible with serum and antibiotics, thus eliminating the need for media change. Protein expression is determined 24 h to 72 h post-transfection.


jetPEI®-Macrophage is perfectly suited for plasmid delivery (DNA, shRNA or miRNA) to cells that express mannose-specific membrane receptors, such as primary macrophages, glial & dendritic cells.

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Every batch of jetPEI®-Macrophage is tested in a transfection assay. Typically, transfection of a firefly luciferase gene under the control of the CMV promote gives 109 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.


To view our protocols, please fill in the fields below and click download.

Other files


Order by :  
Found 72 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
Human primary macrophages, Myeloblastin vitroDNAjetPEI-Macrophage
 20% transfection efficiency
Standard protocol
Human primary macrophages, Myeloblastin vitroDNAjetPEI-Macrophage
107 RLU/mg protein
Standard protocol
Myeloblast, RAW 264.7in vitroDNAjetPEI-Macrophage
15% transfection efficiency
Protocol available
Myeloblast, RAW 264.7in vitroDNAjetPEI-Macrophage
108 RLU/mg protein
Protocol available
Mouse bone marrow-derived denditric cellsin vitroDNA, siRNAINTERFERin, jetPEI-Macrophage
Yang, M. et al. (2011)

J Exp Med 208, 2099-112
E3 ubiquitin ligase CHIP facilitates Toll-like receptor signaling by recruiting and polyubiquitinating Src and atypical PKC{zeta}
More details
Mouse bone marrow-derived macrophagesin vitroDNAjetPEI-Macrophage
Aflaki, E. et al. (2012)

Cell Death Dis 3, e280
C16 ceramide is crucial for triacylglycerol-induced apoptosis in macrophages
More details
HITin vitroDNAjetPEI-Macrophage
Amemiya-Kudo, M. et al. (2005)

J Biol Chem 280, 34577-89
Sterol regulatory element-binding proteins activate insulin gene promoter directly and indirectly through synergy with BETA2/E47
More details
Human monocyte-derived macrophagesin vitroDNAjetPEI-Macrophage
Billiet, L. et al. (2005)

J Biol Chem 280, 40310-8
Extracellular human thioredoxin-1 inhibits lipopolysaccharide-induced interleukin-1beta expression in human monocyte-derived macrophages
More details
Monkey peripheral blood macrophagesin vitroDNAjetPEI-Macrophage
Dhillon, N. K. et al. (2005)

Blood 105, 3094-3099
Inhibition of pathogenic SHIV replication in macaques treated with antisense DNA of interleukin-4
More details
Human peripheral blood mononuclear cellsin vitroDNAjetPEI-Macrophage
Ebisawa, T. et al. (2010)

Neurosci Res 66, 223-7
Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood
More details