Overview

Specifications

Reagent

jetPEI®-Macrophage

Molecule delivered

DNA

Applications

DNA transfection of primary macrophages, glial & dendritic cells

Cell types

Primary macrophages, glial & dendritic cells

Number of transfections

0.5 ml of jetPEI®-Macrophage transfection reagent is sufficient to perform up to 500 transfections in 24-well plates or 160 transfections in 60-mm dishes

Storage

Store jetPEI®-Macrophage at -20°C ± 5°C.
Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.

Provided with

150 mM NaCl


Summary

jetPEI®-Macrophage transfects primary macrophages, glial & dendritic cells that are considered difficult to transfect. It contains a mannose-conjugated linear polyethylenimine that binds to cells expressing mannose-specific membrane receptors, such as macrophages.

jetPEI®-Macrophage is also the reagent of choice for the transfection of cell lines such as RAW 264.7.

jetPEI®-Macrophage is a Mannose-bearing linear polyethylenimine designed to enhance the transfection of cells expressing Mannose receptors on their membrane. Cell targeting is the result of binding of the Mannose residues to the specific cell-surface receptors, leading to internalization of the DNA complexes.

Ordering information

Reference NumberAmount of reagentAmount of NaCl
101000043jetPEI®-Macrophage 0.5 mL50 ml

0.5 ml of jetPEI®-Macrophage transfection reagent is sufficient to perform up to 500 transfections in 24-well plates.

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Description

Transfection of primary human macrophages

jetPEI®-Macrophage allows successful transfection of macrophages derived from monocytes maturated for 7 days in presence of GM-CSF (Fig.1).

jet-PEI-Macrophage-fig1-vc

Fig. 1: Transfection of human macrophages with jetPEI®-Macrophage. Human macrophages expressing Beta-Galactosidase after transfection using jetPEI®-Macrophage.

Higher transfection efficiency compared to versatile jetPEI®

Primary human macrophages and murine RAW 264.7 cells express significantly higher protein levels when transfected with jetPEI®-Macrophage (Fig. 2).

jetPEI-Macrophage - comparison jetPEI

Fig. 2: Comparative efficiency of jetPEI®-Macrophage versus jetPEI® on primary human macrophages and murine RAW 264.7 cells in 24-well plates. Primary human macrophages were transfected in the presence of 100 U/ml GM-CSF and 10% serum, using 1 µg pCMVLuc and 2 µl transfection reagent. Murine RAW 264.7 cells were transfected using 2 µg pCMVLuc and 6.4 µl transfection reagent. Luciferase activity was measured 24 h post-transfection.

Easy to use protocol

The jetPEI®-Macrophage protocol is as simple as the jetPEI® one: Mix the DNA with the reagent to form complexes and simply add the mixture to the cells. jetPEI®-Macrophage is compatible with serum and antibiotics, thus eliminating the need for media change. Protein expression is determined 24 h to 72 h post-transfection.

Applications

jetPEI®-Macrophage is perfectly suited for plasmid delivery (DNA, shRNA or miRNA) to cells that express mannose-specific membrane receptors, such as primary macrophages, glial & dendritic cells.

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Quality

Every batch of jetPEI®-Macrophage is tested in a transfection assay. Typically, transfection of a firefly luciferase gene under the control of the CMV promote gives 109 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.

Protocol

In order to download a product protocol or a certificate of analysis, please create an account on Polyplus-transfection® Portal.

Why would you need to create an account?

In this personal area you will have access to:

  • Product Protocols
  • Certificates of Analysis
  • Exclusive webinars/articles
  • And surprise features!

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Other files

Bibliography

Order by :  
Found 73 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
HITin vitroDNAjetPEI-Macrophage
Amemiya-Kudo, M. et al. (2005)

J Biol Chem 280, 34577-89
Sterol regulatory element-binding proteins activate insulin gene promoter directly and indirectly through synergy with BETA2/E47
More details
Human monocyte-derived macrophagesin vitroDNAjetPEI-Macrophage
Guery, L. et al. (2011)

Blood 118, 4694-704
Fine-tuning nucleophosmin in macrophage differentiation and activation
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Human monocyte-derived macrophagesin vitroDNAjetPEI-Macrophage
Krishnamoorthy, S. et al. (2010)

Proc Natl Acad Sci U S A 107, 1660-5
Resolvin D1 binds human phagocytes with evidence for proresolving receptors
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Human monocyte-derived macrophagesin vitromimic miRNAjetPEI-Macrophage
Recchiuti, A. et al. (2011)

Faseb J 25, 544-60
MicroRNAs in resolution of acute inflammation: identification of novel resolvin D1-miRNA circuits
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RAW 264.7in vitroDNAjetPEI-Macrophage
Zhao, W. et al. (2011)

J Immunol 186, 3173-9
NF-kappaB- and AP-1-mediated DNA looping regulates osteopontin transcription in endotoxin-stimulated murine macrophages
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THP-1in vitroDNAjetPEI-Macrophage
Sikora, A. S. et al. (2016)

J Cell Biochem 117, 1529-42
Regulation of the Expression of Heparan Sulfate 3-O-Sulfotransferase 3B (HS3ST3B) by Inflammatory Stimuli in Human Monocytes
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Mouse alveolar macrophagein vitroDNAjetPEI-Macrophage
Cano, V. et al. (2015)

Cell Microbiol 17, 1537-60
Klebsiella pneumoniae survives within macrophages by avoiding delivery to lysosomes
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Mouse bone marrow-derived macrophagesin vitroDNAjetPEI-Macrophage
Weiss, G. et al. (2013)

MBio 4, e00609-12
Helicobacter pylori VacA suppresses Lactobacillus acidophilus-induced interferon beta signaling in macrophages via alterations in the endocytic pathway
More details
Mouse primary peritoneal macrophages, RAW 264.7in vitroantimiR, antimiR and DNA cotransfection, mimic miRNA, mimic miRNA and DNA cotransfection, siRNAINTERFERin, jetPEI-Macrophage, jetPRIME
Liu, X. et al. (2018)

Cell Mol Immunol 15, 99-110
MicroRNA in vivo precipitation identifies miR-151-3p as a computational unpredictable miRNA to target Stat3 and inhibits innate IL-6 production
More details
Human peripheral blood mononuclear cellsin vitroDNAjetPEI-Macrophage
Ebisawa, T. et al. (2010)

Neurosci Res 66, 223-7
Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood
More details