Overview

Specifications

Reagent

jetPEI®-Macrophage

Molecule delivered

DNA

Applications

DNA transfection of primary macrophages, glial & dendritic cells

Cell types

Primary macrophages, glial & dendritic cells

Number of transfections

0.5 ml of jetPEI®-Macrophage transfection reagent is sufficient to perform up to 500 transfections in 24-well plates or 160 transfections in 60-mm dishes

Storage

5°C ± 3°C, for up to 12 months

Provided with

150 mM NaCl


Summary

jetPEI®-Macrophage transfects primary macrophages, glial & dendritic cells that are considered difficult to transfect. It contains a mannose-conjugated linear polyethylenimine that binds to cells expressing mannose-specific membrane receptors, such as macrophages.

jetPEI®-Macrophage is also the reagent of choice for the transfection of cell lines such as RAW 264.7.

jetPEI®-Macrophage is a Mannose-bearing linear polyethylenimine designed to enhance the transfection of cells expressing Mannose receptors on their membrane. Cell targeting is the result of binding of the Mannose residues to the specific cell-surface receptors, leading to internalization of the DNA complexes.

Ordering information

Reference NumberAmount of reagentAmount of NaCl
103-05N0.5 ml50 ml

0.5 ml of jetPEI®-Macrophage transfection reagent is sufficient to perform up to 500 transfections in 24-well plates.

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Description

Transfection of primary human macrophages

jetPEI®-Macrophage allows successful transfection of macrophages derived from monocytes maturated for 7 days in presence of GM-CSF (Fig.1).

jet-PEI-Macrophage-fig1-vc

Fig. 1: Transfection of human macrophages with jetPEI®-Macrophage. Human macrophages expressing Beta-Galactosidase after transfection using jetPEI®-Macrophage.

Higher transfection efficiency compared to versatile jetPEI®

Primary human macrophages and murine RAW 264.7 cells express significantly higher protein levels when transfected with jetPEI®-Macrophage (Fig. 2).

jetPEI-Macrophage - comparison jetPEI

Fig. 2: Comparative efficiency of jetPEI®-Macrophage versus jetPEI® on primary human macrophages and murine RAW 264.7 cells in 24-well plates. Primary human macrophages were transfected in the presence of 100 U/ml GM-CSF and 10% serum, using 1 µg pCMVLuc and 2 µl transfection reagent. Murine RAW 264.7 cells were transfected using 2 µg pCMVLuc and 6.4 µl transfection reagent. Luciferase activity was measured 24 h post-transfection.

Easy to use protocol

The jetPEI®-Macrophage protocol is as simple as the jetPEI® one: Mix the DNA with the reagent to form complexes and simply add the mixture to the cells. jetPEI®-Macrophage is compatible with serum and antibiotics, thus eliminating the need for media change. Protein expression is determined 24 h to 72 h post-transfection.

Applications

jetPEI®-Macrophage is perfectly suited for plasmid delivery (DNA, shRNA or miRNA) to cells that express mannose-specific membrane receptors, such as primary macrophages, glial & dendritic cells.

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Quality

Every batch of jetPEI®-Macrophage is tested in a transfection assay. Typically, transfection of a firefly luciferase gene under the control of the CMV promote gives 109 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.

Protocol

In order to download a product protocol or a certificate of analysis, please create an account on Polyplus-transfection® Portal.

Why would you need to create an account?

In this personal area you will have access to:

  • Product Protocols
  • Certificates of Analysis
  • Exclusive webinars/articles
  • And surprise features!

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Other files

Bibliography

Order by :  
Found 72 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
Monkey peripheral blood macrophagesin vitroDNAjetPEI-Macrophage
Dhillon, N. K. et al. (2005)

Blood 105, 3094-3099
Inhibition of pathogenic SHIV replication in macaques treated with antisense DNA of interleukin-4
More details
ANA-1in vitroDNAjetPEI-Macrophage
Guo, H. et al. (2008)

J Biol Chem 283, 25209-17
Characterization of short range DNA looping in endotoxin-mediated transcription of the murine inducible nitric-oxide synthase (iNOS) gene
More details
Monkey peripheral blood dendritic cellsin vitroDNAjetPEI-Macrophage
Lisziewicz, J. et al. (2001)

J Virol 75, 7621-8
Induction of potent human immunodeficiency virus type 1-specific T-cell-restricted immunity by genetically modified dendritic cells
More details
Human blood monocyte-derived macrophages, Human monocyte-derived macrophagesin vitrosiRNAjetPEI-Macrophage
Takayama, K. et al. (2006)

Circ Res 98, 499-504
A Novel Prostaglandin E Receptor 4-Associated Protein Participates in Antiinflammatory Signaling
More details
HL-1, RAW 264.7in vitroDNAjetPEI-Macrophage, jetPRIME
Sun, Z. et al. (2016)

Basic Res Cardiol 111, 63
Cross-talk between macrophages and atrial myocytes in atrial fibrillation
More details
Mouse peritoneal macrophagesin vitroDNAjetPEI-Macrophage
Kong, D. et al. (2016)

J Exp Med 213, 2209-26
PKA regulatory IIalpha subunit is essential for PGD2-mediated resolution of inflammation
More details
HEK-293T, Human macrophagesin vitroDNAjetPEI, jetPEI-Macrophage
Mora, J. et al. (2016)

J Mol Cell Biol ,
Interleukin-38 is released from apoptotic cells to limit inflammatory macrophage responses
More details
Mouse bone marrow-derived macrophagesin vitroDNAjetPEI-Macrophage
Hoppstadter, J. et al. (2015)

J Immunol 194, 6057-67
Glucocorticoid-induced leucine zipper: a critical factor in macrophage endotoxin tolerance
More details
RAW 264.7in vitroDNAjetPEI-Macrophage
Schnettger, L. et al. (2017)

Methods Mol Biol 1519, 169-184
Quantitative Spatiotemporal Analysis of Phagosome Maturation in Live Cells
More details
Mouse bone marrow-derived denditric cellsin vitroDNAjetPEI-Macrophage
Ferreira, K. S. et al. (2011)

PLoS ONE 6, e15935
Dendritic cells transfected with scFv from Mab 7.B12 mimicking original antigen gp43 induces protection against experimental Paracoccidioidomycosis
More details