DNA transfection of primary macrophages, glial & dendritic cells
Primary macrophages, glial & dendritic cells
|Number of transfections|
0.5 ml of jetPEI®-Macrophage transfection reagent is sufficient to perform up to 500 transfections in 24-well plates or 160 transfections in 60-mm dishes
5°C ± 3°C, for up to 12 months
150 mM NaCl
jetPEI®-Macrophage transfects primary macrophages, glial & dendritic cells that are considered difficult to transfect. It contains a mannose-conjugated linear polyethylenimine that binds to cells expressing mannose-specific membrane receptors, such as macrophages.
jetPEI®-Macrophage is also the reagent of choice for the transfection of cell lines such as RAW 264.7.
jetPEI®-Macrophage is a Mannose-bearing linear polyethylenimine designed to enhance the transfection of cells expressing Mannose receptors on their membrane. Cell targeting is the result of binding of the Mannose residues to the specific cell-surface receptors, leading to internalization of the DNA complexes.
|Reference Number||Amount of reagent||Amount of NaCl|
|103-05N||0.5 ml||50 ml|
Transfection of primary human macrophages
jetPEI®-Macrophage allows successful transfection of macrophages derived from monocytes maturated for 7 days in presence of GM-CSF (Fig.1).
Fig. 1: Transfection of human macrophages with jetPEI®-Macrophage. Human macrophages expressing Beta-Galactosidase after transfection using jetPEI®-Macrophage.
Higher transfection efficiency compared to versatile jetPEI®
Primary human macrophages and murine RAW 264.7 cells express significantly higher protein levels when transfected with jetPEI®-Macrophage (Fig. 2).
Fig. 2: Comparative efficiency of jetPEI®-Macrophage versus jetPEI® on primary human macrophages and murine RAW 264.7 cells in 24-well plates. Primary human macrophages were transfected in the presence of 100 U/ml GM-CSF and 10% serum, using 1 µg pCMVLuc and 2 µl transfection reagent. Murine RAW 264.7 cells were transfected using 2 µg pCMVLuc and 6.4 µl transfection reagent. Luciferase activity was measured 24 h post-transfection.
Easy to use protocol
The jetPEI®-Macrophage protocol is as simple as the jetPEI® one: Mix the DNA with the reagent to form complexes and simply add the mixture to the cells. jetPEI®-Macrophage is compatible with serum and antibiotics, thus eliminating the need for media change. Protein expression is determined 24 h to 72 h post-transfection.
jetPEI®-Macrophage is perfectly suited for plasmid delivery (DNA, shRNA or miRNA) to cells that express mannose-specific membrane receptors, such as primary macrophages, glial & dendritic cells.
Every batch of jetPEI®-Macrophage is tested in a transfection assay. Typically, transfection of a firefly luciferase gene under the control of the CMV promote gives 109 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.
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|Cell Line||in vitro|
|Delivered Molecule||Reagent||Results & Citations|
|Monkey peripheral blood macrophages||in vitro||DNA||jetPEI-Macrophage|
Dhillon, N. K. et al. (2005)
Blood 105, 3094-3099
Inhibition of pathogenic SHIV replication in macaques treated with antisense DNA of interleukin-4
Guo, H. et al. (2008)
J Biol Chem 283, 25209-17
Characterization of short range DNA looping in endotoxin-mediated transcription of the murine inducible nitric-oxide synthase (iNOS) gene
|Monkey peripheral blood dendritic cells||in vitro||DNA||jetPEI-Macrophage|
Lisziewicz, J. et al. (2001)
J Virol 75, 7621-8
Induction of potent human immunodeficiency virus type 1-specific T-cell-restricted immunity by genetically modified dendritic cells
|Human blood monocyte-derived macrophages, Human monocyte-derived macrophages||in vitro||siRNA||jetPEI-Macrophage|
Takayama, K. et al. (2006)
Circ Res 98, 499-504
A Novel Prostaglandin E Receptor 4-Associated Protein Participates in Antiinflammatory Signaling
|HL-1, RAW 264.7||in vitro||DNA||jetPEI-Macrophage, jetPRIME|
Sun, Z. et al. (2016)
Basic Res Cardiol 111, 63
Cross-talk between macrophages and atrial myocytes in atrial fibrillation
|Mouse peritoneal macrophages||in vitro||DNA||jetPEI-Macrophage|
Kong, D. et al. (2016)
J Exp Med 213, 2209-26
PKA regulatory IIalpha subunit is essential for PGD2-mediated resolution of inflammation
|HEK-293T, Human macrophages||in vitro||DNA||jetPEI, jetPEI-Macrophage|
Mora, J. et al. (2016)
J Mol Cell Biol ,
Interleukin-38 is released from apoptotic cells to limit inflammatory macrophage responses
|Mouse bone marrow-derived macrophages||in vitro||DNA||jetPEI-Macrophage|
Hoppstadter, J. et al. (2015)
J Immunol 194, 6057-67
Glucocorticoid-induced leucine zipper: a critical factor in macrophage endotoxin tolerance
|RAW 264.7||in vitro||DNA||jetPEI-Macrophage|
Schnettger, L. et al. (2017)
Methods Mol Biol 1519, 169-184
Quantitative Spatiotemporal Analysis of Phagosome Maturation in Live Cells
|Mouse bone marrow-derived denditric cells||in vitro||DNA||jetPEI-Macrophage|
Ferreira, K. S. et al. (2011)
PLoS ONE 6, e15935
Dendritic cells transfected with scFv from Mab 7.B12 mimicking original antigen gp43 induces protection against experimental Paracoccidioidomycosis