Overview

Specifications

Reagent

jetPEI®-Hepatocyte

Molecule delivered

DNA

Applications

DNA transfection of primary hepatocytes

Cell types

Primary hepatocytes

Number of transfections

0.5 ml of jetPEI®-Hepatocyte transfection reagent is sufficient to perform up to 150 transfections in 24-well plates or 30 transfections in 60-mm plates

Storage

5°C ± 3°C, for up to 12 months

Provided with

150 mM NaCl


Summary

jetPEI®-Hepatocyte is a DNA transfection reagent designed to transfect Hepatocyte-like cells. jetPEI®-Hepatocyte is recommended to transfect primary hepatocytes and cell lines such as human hepatocarcinoma Hep G2 and murine hepatocytes BNL-CL.2.

jetPEI®-Hepatocyte is a Galactose-bearing linear polyethylenimine designed to enhance the transfection of cells expressing Galactose-specific membrane lectins, such as hepatocytes that express the asialoglycoprotein receptor (ASGP-R or Gal/GalNAc receptor). Cell targeting is the result of binding of the Galactose residues to the specific cell-surface receptors, leading to internalization of the DNA complexes.

Ordering information

Reference NumberAmount of reagentAmount of NaCl
102-05N0.5 ml50 ml

0.5 ml of jetPEI®-Hepatocyte transfection reagent is sufficient to perform ca. 150 transfections in 24-well plates.

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Description

jetPEI®-Hepatocyte leads to improved transfection efficiency of hepatocarcinoma Hep G2 cells

Compared to our standard jetPEI® transfection reagent, jetPEI®-Hepatocyte provides significantly higher transfection efficiencies in human hepatocarcinoma Hep G2 cells as well as in difficult to transfect primary human hepatocytes (Fig. 1).

jetPEI-Hepatocyte - Comparison jetPEI

Fig. 1: Comparative transfection efficiency of jetPEI®-Hepatocyte versus jetPEI®. Hep G2 (50,000 cells in 24-w) and primary human hepatocytes (100,000 cells in 24-w) were transfected using 3.2 µl of both reagents with 1 µg of pCMVEGFPLuc, in 1 ml of complete medium containing 10% of serum. The luciferase activity was determined 48h post-transfection. Transfection efficiency was expressed as relative efficiency obtained with jetPEI® compared to jetPEI®-Hepatocyte.

Good transfection efficiency and excellent viability in primary hepatocytes

Primary human hepatocytes show good transfection efficiency (around 50%) 24 h and 48 h after transfection using jetPEI®-Hepatocyte and luciferase plasmid pCMVEGFPLuc. In addition, cells look very healthy (Fig. 2).

jetPEI hepatocyte - primary human hepatocytes

Fig. 2: Primary human hepatocytes expressing GFP 24 h and 48 h after transfection using jetPEI®-Hepatocyte. Primary human hepatocytes (100,000 cells in 24-w) were transfected with 1 µg of pCMVEGFPLuc at N/P ratio of 8, in 1 ml of complete medium containing 10% of serum. Cells were visualized by fluorescence microscopy.

Easy transfection of hepatocyte-derived cell lines

The most popular hepatocyte-derived cell lines BNL-CL.2 and Hep G2 were successfully transfected using jetPEI®-Hepatocyte and commonly show approximately 50 % and 30% transfection efficiency, respectively (Fig. 3 and 4).

jetPEI hepatocyte - BNLCL2 cells

Fig. 3: BNL-CL.2 cells expressing ß-galactosidase after transfection using jetPEI®-Hepatocyte. BNL-CL.2 cells (50,000 cells in 24-w) were transfected using 3.2 µl of reagent with 1 µg of pCMV-betaGal, in 1 ml of complete medium containing 10% of serum. ß-galactosidase gene expression was visualized by X-gal staining after 24h.

jetPEI hepatocyte - HepG2 cells

Fig. 4: Hep G2 cells expressing GFP 72 h after transfection using jetPEI®-Hepatocyte. Hep G2 cells (50,000 cells in 24-w) were transfected using 3.2 µl of reagent with 1 µg of pCMVEGFPLuc, in 1 ml of complete medium containing 10% of serum. Cells expressing GFP protein were visualized by fluorescence.

Easy to use protocol

The jetPEI®-Hepatocyte protocol is as simple as the jetPEI® one: Mix the DNA with the reagent to form complexes and simply add the mixture to the cells. jetPEI®-Hepatocyte is compatible with serum and antibiotics, thus eliminating the need for media changes. Protein expression is determined 24 h to 72 h post-transfection.

Applications

jetPEI®-Hepatocyte is perfectly suited for plasmid delivery (DNA, shRNA or miRNA) to primary hepatocytes as well as hepatocarcinoma cells, such as Hep G2 and BNL-CL.2.

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Quality

Every batch of jetPEI®-Hepatocyte is tested in a transfection assay. Typically, transfection of a firefly luciferase gene under the control of the CMV promoter gives 109 RLU (relative light unit)/mg of protein. The value for each batch is indicated on the Certificate of Analysis.

Protocol

In order to download a product protocol or a certificate of analysis, please create an account on Polyplus-transfection® Portal.

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  • Product Protocols
  • Certificates of Analysis
  • Exclusive webinars/articles
  • And surprise features!

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Other files

Bibliography

Order by :  
Found 32 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
Rat primary hepatocytesin vitroshRNA plasmidjetPEI-Hepatocyte
Naimi, M. et al. (2007)

Endocrinology 148, 2424-34
Nuclear forkhead box O1 controls and integrates key signaling pathways in hepatocytes
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Hep G2, PLC/PRF/5in vitroDNA, siRNAINTERFERin, jetPEI-Hepatocyte
Kessler, S. M. et al. (2013)

Am J Physiol Gastrointest Liver Physiol 304, G328-36
IGF2 mRNA binding protein p62/IMP2-2 in hepatocellular carcinoma: antiapoptotic action is independent of IGF2/PI3K signaling
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Polishchuk, E. V. et al. (2014)

Dev Cell 29, 686-700
Wilson disease protein ATP7B utilizes lysosomal exocytosis to maintain copper homeostasis
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HepaRGin vitroDNAjetPEI-Hepatocyte
Andrieux, L. O. et al. (2007)

Cancer Res 67, 2114-23
GATA-1 is essential in EGF-mediated induction of nucleotide excision repair activity and ERCC1 expression through ERK2 in human hepatoma cells
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Arima, H. et al. (2012)

Pharmaceutics 4, 130-48
Polyamidoamine Dendrimer Conjugates with Cyclodextrins as Novel Carriers for DNA, shRNA and siRNA
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Hep G2in vitroDNAjetPEI-Hepatocyte
Makkonen, K. E. et al. (2013)

J Virol 87, 11148-59
6-o- and N-sulfated syndecan-1 promotes baculovirus binding and entry into Mammalian cells
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Hep G2in vitroDNAjetPEI-Hepatocyte
Benoki, S. et al. (2012)

Arch Biochem Biophys 517, 123-30
Transactivation of ABCG2 through a novel cis-element in the distal promoter by constitutive androstane receptor but not pregnane X receptor in human hepatocytes
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Hep3B, PLC/PRF/5in vitroDNAjetPEI-Hepatocyte
Fu, X. et al. (2018)

Mol Cancer 17, 73
Linc00210 drives Wnt/beta-catenin signaling activation and liver tumor progression through CTNNBIP1-dependent manner
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Human primary hepatocytesin vitroDNAjetPEI-Hepatocyte
Zhang, J. et al. (2018)

Cell Host Microbe 23, 819-831 e5
Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production
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COS-7, Hep3Bin vitroDNAjetPEI, jetPEI-Hepatocyte
Takahashi, E. et al. (2014)

Am J Physiol Cell Physiol 306, C334-42
Anaerobic respiration sustains mitochondrial membrane potential in a prolyl hydroxylase pathway-activated cancer cell line in a hypoxic microenvironment
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