Overview
Specifications
| Reagent | jetOPTIMUS® DNA Transfection Reagent |
|---|---|
| Molecule delivered | Plasmid DNA |
| Applications | Transient and stable gene expression from plasmid DNA transfection |
| Cell types | Adherent hard-to-transfect mammalian cell: |
| Number of transfections | 1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol |
| Storage | 5°C ± 3°C, stable for at least one year when stored appropriately |
| Provided with | jetOPTIMUS® Buffer |
Summary
Ace your DNA transfection in the cell model you need!
As Transfection Experts, we know that it is essential for biologists to work with physiologically relevant cell models. For gene expression studies, transfection is the preferred technique to introduce a gene of interest in a given cell model, due to its cost-effectiveness and simplicity-of-use in comparison to physical techniques (e.g. micro-injection, electroporation).
Several types of cells (primary cells, stem cells or cancer cell lines) remain difficult to transfect with DNA for different reasons: slow-dividing rates, cell fragility and cellular defense mechanisms. To address the current limits of DNA transfection and complement our existing gene expression portfolio (jetPEI®, jetPRIME®, jetMESSENGER®), Polyplus-transfection® engineered an innovative delivery nanoparticle: jetOPTIMUS®.
jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency. In order to work in relevant physiological conditions, transfection with jetOPTIMUS® requires a minimum DNA quantity and reagent volume to keep an excellent cell viability and morphology.
Ordering information
| Reference number | Reagent size | Buffer size |
|---|---|---|
| 117-01 | 0.1 mL | 10 mL |
| 117-07 | 0.75 mL | 2 x 60 mL |
| 117-15 | 1.5 mL | 4 x 60 mL |
| 717-60 | x | 60 mL |
Description
Optimized DNA transfection efficiency on hard-to-transfect cells
jetOPTIMUS® is an innovative cationic nanotechnology developed to improve DNA transfection efficiency in easy- and difficult-to-transfect cells used as in vitro cell culture models. Tested on various primary cells and cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression than main competitors.
Fig. 1: jetOPTIMUS® outperforms its main competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection with a plasmid coding for a GFP protein in 24-well plates. Conditions were set up according to the manufacturer’s recommendations both for Lipofectamine® 3000 and for jetOPTIMUS®.
Advanced nanotechnology: Superior gene expression using less material!
jetOPTIMUS® has been developed to increase transfection efficiency into a wide range of cell types, while keeping the amount of DNA as low as possible, and reducing the volume of reagent required compared to main competitors. Consequently, scientists can increase the number of transfections and reduce the cost per reaction by up to seven-fold (table 1).
Table 1: Lower volume of jetOPTIMUS® is required compared to Lipofectamine® 3000 in 24-well plate.
Excellent cell viability and morphology
Reaching high transfection efficiencies is often at the expense of cellular integrity, with adverse effects on cell cycle, metabolism and signaling pathways. At Polyplus-transfection, we make no compromise on cell integrity: jetOPTIMUS® transfection reagent is gentle on cells, allowing generation of biologically relevant data from gene expression studies.
Fig. 2: Cells remains healthy and keep a good morphology 24h after transfection. Phase contrast microscopy of both MDCK and VERO cells 24 h after transfections with a plasmid coding for a GFP protein performed according to the manufacturer’s recommendations for each reagent.
Optimized transfection protocol
jetOPTIMUS® is a ready-to-use transfection reagent provided with its own complexation buffer (jetOPTIMUS® Buffer). The protocol is optimized for simplicity of use (all plate sizes), culture medium compatibility (antibiotics, serum) and cost-effectiveness (lowest amount of DNA and volume of reagent).
Fig. 3: Simplicity of the jetOPTIMUS® protocol for DNA transfection, here in 24-well plates.
FAQ
If you have any questions regarding jetOPTIMUS®, please visit our dedicated Frequently asked questions or contact us.
Applications
DNA transfection
Plasmids are small circular DNA molecules that are commonly found in bacteria. Plasmids exist and replicate separately from chromosomal DNA and in bacteria they often carry genes that are beneficial for bacterial survival. Plasmids can be deliberately introduced into desired cells and utilized to overexpress a gene of interest in a specific cell line. This procedure is called DNA transfection and is a commonly used method for studying gene function or protein of interest.
CRISPR genome editing
The use of the CRISPR/Cas9 system in mammalian cells has recently emerged as a very convenient way to modify the cell genome at a specific locus. It involves transient transfection into mammalian cells of either (a) one or several plasmids coding for Cas9, the specific gRNA and eventually the sequence to be inserted, or (b) a mix of one or two plasmids and an RNA molecule (the gRNA).
Citations
Force-induced recruitment of cten along keratin network in epithelial cells. Joleen S. Cheah, Kyle A. Jacobs, Volkmar Heinrich, Su Hao Lo, Soichiro Yamada. Proceedings of the National Academy of Sciences Oct 2019, 116 (40) 19799-19801; DOI: 10.1073/pnas.1911865116
Targeting IDH1 as a pro-senescent therapy in high-grade serous ovarian cancer. Erika S Dahl, Raquel Buj, Kelly E Leon, Jordan M Newell, Yuka Imamura, Benjamin G. Bitler, Nathaniel W Snyder and Katherine M Aird.Mol Cancer Res May 20 2019 DOI: 10.1158/1541-7786.MCR-18-1233
Quality
Each lot of jetOPTIMUS® is tested in-house by DNA transfection in HeLa cells following standard protocol. Every batch will be provided with Certificate of Analysis.
Polyplus-transfection® is ISO 9001 Quality Management System accredited since 2002; this level of certification assures global customers that the supplier has established reliable and effective processes for product development, manufacturing, sales and customer support.
Testimonials
Bibliography
| Cell Line | in vitro in vivo | Delivered Molecule | Reagent | Results & Citations | |
|---|---|---|---|---|---|
| HEK-293 | in vitro | DNA | jetOPTIMUS | 70-90% transfection efficiency | Protocol available |
| HeLa | in vitro | DNA | jetOPTIMUS | 70-90% transfection efficiency | Protocol available |
| Caco-2 | in vitro | DNA | jetOPTIMUS | 30-50 % transfection efficiency | Protocol available |
| Hep G2 | in vitro | DNA | jetOPTIMUS | 50-70% Transfection efficiency | Protocol available |
| MCF 10A | in vitro | DNA | jetOPTIMUS | 20-40% transfection efficiency | Protocol available |
| MDCK | in vitro | DNA | jetOPTIMUS | 30-50 % transfection efficiency | Protocol available |
| VERO | in vitro | DNA | jetOPTIMUS | 70-90 % Transfection efficiency | Protocol available |
| MCF7 | in vitro | DNA | jetOPTIMUS | 50-70 % Transfection efficiency | Protocol available |
| A549 | in vitro | DNA | jetOPTIMUS | 40-60% transfection efficiency | Protocol available |
| N2A | in vitro | DNA | jetOPTIMUS | 70-90% transfection efficiency | Protocol available |