Overview
Specifications
| Reagent | jetOPTIMUS® DNA Transfection Reagent |
|---|---|
| Molecule delivered | Plasmid DNA |
| Applications | Transient and stable gene expression from plasmid DNA transfection |
| Cell types | Adherent hard-to-transfect mammalian cell: |
| Number of transfections | 1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol |
| Storage | 5°C ± 3°C, stable for at least one year when stored appropriately |
| Provided with | jetOPTIMUS® Buffer |
Summary
®Ace your DNA transfection in the cell model you need!
As Transfection Experts, we know that it is essential for biologists to work with physiologically relevant cell models. For gene expression studies, transfection is the preferred technique to introduce a gene of interest in a given cell model, due to its cost-effectiveness and simplicity-of-use in comparison to physical techniques (e.g. micro-injection, electroporation).
Several types of cells (primary cells, stem cells or cancer cell lines) remain difficult to transfect with DNA for different reasons: slow-dividing rates, cell fragility and cellular defense mechanisms. To address the current limits of DNA transfection and complement our existing gene expression portfolio (jetPEI®, jetPRIME®, jetMESSENGER®), Polyplus-transfection® engineered an innovative delivery nanoparticle: jetOPTIMUS®.
jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency. In order to work in relevant physiological conditions, transfection with jetOPTIMUS® requires a minimum DNA quantity and reagent volume to keep an excellent cell viability and morphology.
Ordering information
| Reference number | Reagent size | Buffer size |
|---|---|---|
| 117-01 | 0.1 mL | 10 mL |
| 117-07 | 0.75 mL | 2 x 60 mL |
| 117-15 | 1.5 mL | 4 x 60 mL |
| 717-60 | x | 60 mL |
Description
Optimized DNA transfection efficiency on hard-to-transfect cells
jetOPTIMUS® is an innovative cationic nanotechnology developed to improve DNA transfection efficiency in easy- and difficult-to-transfect cells used as in vitro cell culture models. Tested on various primary cells and cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression than main competitors.
Fig. 1: jetOPTIMUS® outperforms its main competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection with a plasmid coding for a GFP protein in 24-well plates. Conditions were set up according to the manufacturer’s recommendations both for Lipofectamine® 3000 and for jetOPTIMUS®.
Advanced nanotechnology: Superior gene expression using less material!
jetOPTIMUS® has been developed to increase transfection efficiency into a wide range of cell types, while keeping the amount of DNA as low as possible, and reducing the volume of reagent required compared to main competitors. Consequently, scientists can increase the number of transfections and reduce the cost per reaction by up to seven-fold (table 1).
Table 1: Lower volume of jetOPTIMUS® is required compared to Lipofectamine® 3000 in 24-well plate.
Excellent cell viability and morphology
Reaching high transfection efficiencies is often at the expense of cellular integrity, with adverse effects on cell cycle, metabolism and signaling pathways. At Polyplus-transfection, we make no compromise on cell integrity: jetOPTIMUS® transfection reagent is gentle on cells, allowing generation of biologically relevant data from gene expression studies.
Fig. 2: Cells remains healthy and keep a good morphology 24h after transfection. Phase contrast microscopy of both MDCK and VERO cells 24 h after transfections with a plasmid coding for a GFP protein performed according to the manufacturer’s recommendations for each reagent.
Optimized transfection protocol
jetOPTIMUS® is a ready-to-use transfection reagent provided with its own complexation buffer (jetOPTIMUS® Buffer). The protocol is optimized for simplicity of use (all plate sizes), culture medium compatibility (antibiotics, serum) and cost-effectiveness (lowest amount of DNA and volume of reagent).
Fig. 3: Simplicity of the jetOPTIMUS® protocol for DNA transfection, here in 24-well plates.
FAQ
If you have any questions regarding jetOPTIMUS®, please visit our dedicated Frequently asked questions or contact us.
Applications
DNA transfection
Plasmids are small circular DNA molecules that are commonly found in bacteria. Plasmids exist and replicate separately from chromosomal DNA and in bacteria they often carry genes that are beneficial for bacterial survival. Plasmids can be deliberately introduced into desired cells and utilized to overexpress a gene of interest in a specific cell line. This procedure is called DNA transfection and is a commonly used method for studying gene function or protein of interest.
📰 Available Application Notes:
Improved gene expression in hard-to-transfect cells with jetOPTIMUS® Transfection Reagent
CRISPR genome editing
The use of the CRISPR/Cas9 system in mammalian cells has recently emerged as a very convenient way to modify the cell genome at a specific locus. It involves transient transfection into mammalian cells of either (a) one or several plasmids coding for Cas9, the specific gRNA and eventually the sequence to be inserted, or (b) a mix of one or two plasmids and an RNA molecule (the gRNA).
Virus production
jetOPTIMUS® transfection reagent is highly effective for routine virus production of both AAV and lentivirus in adherent cells grown in classical media such as DMEM in presence of serum.
Quality
Each lot of jetOPTIMUS® is tested in-house by DNA transfection in HeLa cells following standard protocol. Every batch will be provided with Certificate of Analysis.
Polyplus-transfection® is ISO 9001 Quality Management System accredited since 2002; this level of certification assures global customers that the supplier has established reliable and effective processes for product development, manufacturing, sales and customer support.
Testimonials
Protocol
To view our protocols, please fill in the fields below and click download.
Other files
Related blog posts
Bibliography
| Cell Line | in vitro in vivo | Delivered Molecule | Reagent | Results & Citations | |
|---|---|---|---|---|---|
| OVCAR-3 | in vitro | DNA | jetOPTIMUS | Dahl, E. S. et al. (2019) Mol Cancer Res , Targeting IDH1 as a Prosenescent Therapy in High-grade Serous Ovarian Cancer | More details |
| PC-3 | in vitro | DNA | jetOPTIMUS | Baker MJ. et al. (2020 ) J Biol Chem , Evaluation of active Rac1 levels in cancer cells: a case of misleading conclusions from immunofluorescence analysis | More details |
| HEK-293T | in vitro | DNA | jetOPTIMUS | Moparthi L. et al. (2020) Differentiation 115, 30-36 A uniform expression library for the exploration of FOX transcription factor biology | More details |
| HeLa | in vitro | DNA | jetOPTIMUS | Königs V. et al. (2020 ) Nat Struct Mol Biol 27, 260-273 SRSF7 maintains its homeostasis through the expression of Split-ORFs and nuclear body assembly | More details |
| Hep G2 | in vitro | DNA | jetOPTIMUS | D'Amore C. et al. (2020 ) BBA Mol Cell Res 1867, 118807 “Janus” efficacy of CX-5011: CK2 inhibition and methuosis induction by independent mechanisms | More details |
| BHK-21, HEK-293T, VERO | in vitro | DNA | jetOPTIMUS | Marano JM. et al. (2020) Virology 551, 58-63 Rolling circle amplification: A high fidelity and efficient alternative to plasmid preparation for the rescue of infectious clones | More details |
| HEK-293, HeLa | in vitro | DNA | jetOPTIMUS | Lee, WK. et al. (2020) Hum. Mutat. 41, 1321-1328 Disease‐causing missense mutations within the N‐terminal transmembrane domain of GlcNAc‐1‐phosphotransferase impair endoplasmic reticulum translocation or Golgi retention | More details |
| CT26 | in vitro | DNA | jetOPTIMUS | Choi SS. et al. (2020) Exp Mol Med , Flightless-1 inhibits ER stress-induced apoptosis in colorectal cancer cells by regulating Ca2+ homeostasis | More details |
| HEK-293 | in vitro | DNA | jetOPTIMUS | Elbasani E. et al. (2020) Viruses 12, 952 Kaposi’s Sarcoma-Associated Herpesvirus Reactivation by Targeting of a dCas9-Based Transcription Activator to the ORF50 Promoter | More details |
| OVCAR-3 | in vitro | DNA | jetOPTIMUS | Dahl E. et al. (2020) Mol Cancer Res , Targeting IDH1 as a Prosenescent Therapy in High-grade Serous Ovarian Cancer | More details |