Overview

Specifications

Reagent

jetOPTIMUS® DNA Transfection Reagent

Molecule delivered

Plasmid DNA

Applications

Transient and stable gene expression from plasmid DNA transfection
CRISPR Genome editing using DNA approach

Cell types

Adherent hard-to-transfect mammalian cell:
- Primary cells (epithelial, hepatocyte, endothelial, fibroblast, etc…)
- Stem cells (embryonic, mesenchymal, induced pluripotent)
- Cancer cell lines

Number of transfections

1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol

Storage

5°C ± 3°C, stable for 6 months (0.1 mL) to at at least one year (other packaging sizes) when stored appropriately

Provided with

jetOPTIMUS® Buffer


Summary

®Ace your DNA transfection in the cell model you need!

As Transfection Experts, we know that it is essential for biologists to work with physiologically relevant cell models. For gene expression studies, transfection is the preferred technique to introduce a gene of interest in a given cell model, due to its cost-effectiveness and simplicity-of-use in comparison to physical techniques (e.g. micro-injection, electroporation).

Several types of cells (primary cells, stem cells or cancer cell lines) remain difficult to transfect with DNA for different reasons: slow-dividing rates, cell fragility and cellular defense mechanisms. To address the current limits of DNA transfection and complement our existing gene expression portfolio (jetPEI®, jetPRIME®, jetMESSENGER®), Polyplus-transfection® engineered an innovative delivery nanoparticle: jetOPTIMUS®.

jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency. In order to work in relevant physiological conditions, transfection with jetOPTIMUS® requires a minimum DNA quantity and reagent volume to keep an excellent cell viability and morphology.

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Ordering information

Reference numberReagent sizeBuffer size
101000051jetOPTIMUS® 0.1 mL10 mL
101000025jetOPTIMUS® 0.75 mL2 x 60 mL
101000006jetOPTIMUS® 1.5 mL4 x 60 mL
201000001xjetOPTIMUS® Buffer 60 mL

Bulk quantities are available upon request. Please contact us.

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Description

Optimized DNA transfection efficiency on hard-to-transfect cells

jetOPTIMUS® is an innovative cationic nanotechnology developed to improve DNA transfection efficiency in easy- and difficult-to-transfect cells used as in vitro cell culture models. Tested on various primary cells and cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression than main competitors.

jetOPTIMUS - Maximal transfection efficiency

Fig. 1: jetOPTIMUS® outperforms its main competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection with a plasmid coding for a GFP protein in 24-well plates. Conditions were set up according to the manufacturer’s recommendations both for Lipofectamine® 3000 and for jetOPTIMUS®.

Advanced nanotechnology: Superior gene expression using less material!

jetOPTIMUS® has been developed to increase transfection efficiency into a wide range of cell types, while keeping the amount of DNA as low as possible, and reducing the volume of reagent required compared to main competitors. Consequently, scientists can increase the number of transfections and reduce the cost per reaction by up to seven-fold (table 1).

jetOPTIMUS - Cost-effectiveness

Table 1: Lower volume of jetOPTIMUS® is required compared to Lipofectamine® 3000 in 24-well plate.

Excellent cell viability and morphology

Reaching high transfection efficiencies is often at the expense of cellular integrity, with adverse effects on cell cycle, metabolism and signaling pathways. At Polyplus-transfection, we make no compromise on cell integrity: jetOPTIMUS® transfection reagent is gentle on cells, allowing generation of biologically relevant data from gene expression studies.

jetOPTIMUS - Cell Viability

Fig. 2: Cells remains healthy and keep a good morphology 24h after transfection. Phase contrast microscopy of both MDCK and VERO cells 24 h after transfections with a plasmid coding for a GFP protein performed according to the manufacturer’s recommendations for each reagent.

Optimized transfection protocol

jetOPTIMUS® is a ready-to-use transfection reagent provided with its own complexation buffer (jetOPTIMUS® Buffer). The protocol is optimized for simplicity of use (all plate sizes), culture medium compatibility (antibiotics, serum) and cost-effectiveness (lowest amount of DNA and volume of reagent).

jetOPTIMUS - Protocol

Fig. 3: Simplicity of the jetOPTIMUS® protocol for DNA transfection, here in 24-well plates.

FAQ

If you have any questions regarding jetOPTIMUS®, please visit our dedicated Frequently asked questions or contact us.

Applications

DNA transfection

Plasmids are small circular DNA molecules that are commonly found in bacteria. Plasmids exist and replicate separately from chromosomal DNA and in bacteria they often carry genes that are beneficial for bacterial survival. Plasmids can be deliberately introduced into desired cells and utilized to overexpress a gene of interest in a specific cell line. This procedure is called DNA transfection and is a commonly used method for studying gene function or protein of interest.

📰 Available Application Notes:

Improved gene expression in hard-to-transfect cells with jetOPTIMUS® Transfection Reagent

Read more…

CRISPR genome editing

The use of the CRISPR/Cas9 system in mammalian cells has recently emerged as a very convenient way to modify the cell genome at a specific locus. It involves transient transfection into mammalian cells of either (a) one or several plasmids coding for Cas9, the specific gRNA and eventually the sequence to be inserted, or (b) a mix of one or two plasmids and an RNA molecule (the gRNA).

📰 Download our Researcher’s guide to Genome editing!

Read more…

Virus production

jetOPTIMUS® transfection reagent is highly effective for routine virus production of both AAV and lentivirus in adherent cells grown in classical media such as DMEM in presence of serum.

Read more…

Quality

Each lot of jetOPTIMUS® is tested in-house by DNA transfection in HeLa cells following standard protocol. Every batch will be provided with Certificate of Analysis.

Polyplus-transfection® is ISO 9001 Quality Management System accredited since 2002; this level of certification assures global customers that the supplier has established reliable and effective processes for product development, manufacturing, sales and customer support.

Testimonials

I have tested many different transfection reagents during the past 15 years and I have never been as impressed as I was today by the performance of jetOPTIMUS. Excellent viability at 24h and remarkable transfection efficiency (pEGFP-C1). The 100 µl free sample was generous and we will evaluate it further in our experiments during the coming month. If results continue to look this good we will definitely order the reagent
 Helena P., University of Lund , SE 
  
"There is a massive improvement with the jetOPTIMUS® compared with gold standard Lipofectamine. Something like 30% more transfected cells, no mortality."
 Gautier F., University of Turku, FI 
 Postdoctoral Researcher 
"Our lab recently started using jetOPTIMUS® for our transfection work as we were needing to improve transfection efficiency. We found that JetOPTIMUS was superior to other tested reagents in all of the cell lines we tested."
 Zach T., Case Western Reserve University, Cleveland, OH 
  
"I used the jetOPTIMUS® for CRISPR-Cas9 experiments in HT-1080 cell line and this reagent worked very well with reproducible results. I obtained an improved INDEL percentage with jetOPTIMUS® (60%) when compared to nucleofection with Amaxa (53%) in experiments performed in parallel. Finally, I was able to reach 4% knock in efficiency."
 Laurent M., Centre de Recherches en Cancérologie de Toulouse, FR 
  
"Where other methods have failed, jetOPTIMUS® works in transfecting primary fibroblast cultures with high transfection efficiency and low toxicity"
 Marlene J., Temple University, US 
  
“In comparison to another transfection reagent, jetOPTIMUS® was mind-blowingly better for the difficult to transfect 3T3-NR6 cells!“
 Danai L., Katholieke Universiteit Leuven, BE 
  
"Very easy to use, sensitive cell lines support it very well, good efficiency and reproducibility, I would recommend it."
 Huguette D., UZH, CH 
  
"We have just had a chance to try jetOPTIMUS® and we observed at least 60% percent transfection efficiency even in cells which are hard to transfect. Great product you should try."
 Ari Uyar O., Gebze Technical University, TR 
  
"I tried both jetOPTIMUS® and jetPRIME® transfection reagents on SH-SY5Y cells, both worked very well as I observed approximately 90% transfection efficiency. I would recommend it."
 Ofek O., Ben Gurion University of the negev, IL 
  
“jetOPTIMUS® is a great product. It worked better than competitors to deliver large plasmid constructs into hard-to-transfect cells."
 Mona S., University, DE 
  

Bibliography

Order by :  
Found 59 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
Hep G2in vitroDNAjetOPTIMUS
D'Amore C. et al. (2020 )

BBA Mol Cell Res 1867, 118807
“Janus” efficacy of CX-5011: CK2 inhibition and methuosis induction by independent mechanisms
More details
BHK-21, HEK-293T, VEROin vitroDNAjetOPTIMUS
Marano JM. et al. (2020)

Virology 551, 58-63
Rolling circle amplification: A high fidelity and efficient alternative to plasmid preparation for the rescue of infectious clones
More details
A375, Hep-3Bin vitroDNAjetOPTIMUS
De Backer J. et al. (2021)

Sci Rep 11(1),
A reliable set of reference genes to normalize oxygen-dependent cytoglobin gene expression levels in melanoma
More details
HEK-293Tin vitroDNAjetOPTIMUS
Troyer Z. et al. (2021)

J Extracell Vesicles 10,
Extracellular vesicles carry SARS-CoV-2 spike protein and serve as decoys for neutralizing antibodies
More details
HEK-293in vitroDNAjetOPTIMUS, jetPRIME
Ufartes R. et al. (2021)

Hum Mol Genet ,
CHARGE syndrome and related disorders: A mechanistic link
More details
HEK-293, HeLain vitroDNAjetOPTIMUS
Lee, WK. et al. (2020)

Hum. Mutat. 41, 1321-1328
Disease‐causing missense mutations within the N‐terminal transmembrane domain of GlcNAc‐1‐phosphotransferase impair endoplasmic reticulum translocation or Golgi retention
More details
CT26in vitroDNAjetOPTIMUS
Choi SS. et al. (2020)

Exp Mol Med ,
Flightless-1 inhibits ER stress-induced apoptosis in colorectal cancer cells by regulating Ca2+ homeostasis
More details
HEK-293Tin vitroDNAjetOPTIMUS
Zhang K. et al. (2021)

Sci Adv 7, eabe7386
Nsp1 protein of SARS-CoV-2 disrupts the mRNA export machinery to inhibit host gene expression
More details
HEK-293, MEF, SH-SY5Yin vitroDNAjetOPTIMUS, jetPRIME
Ramesh G. et al. (2021)

Cell Rep 34(11),
A short isoform of STIM1 confers frequency-dependent synaptic enhancement
More details
CHO-ldlD, CHO-pro5in vitroDNAjetOPTIMUS
Zhu J. et al. (2021)

J Mol Cell Cardiol 154, 70-79
β 1-adrenergic receptor N-terminal cleavage by ADAM17; the mechanism for redox-dependent downregulation of cardiomyocyte β 1-adrenergic receptors
More details