Molecule delivered



Gene expression
Genome Editing
Stem cell differentiation

Cell types

Hard-to-transfect cells
Adherent and suspension cells
Primary cells (Neurons, Stem cells, Immune cells, fibroblasts, …)
Post-mitotic cells

Number of transfections

1.5 ml of jetMESSENGER® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates


Store jetMESSENGER® at 5 °C ± 3°C.
Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.

Provided with

mRNA buffer


Take your gene expression to the next level by switching to mRNA!

Primary cells, neurons, suspension cells and various cancer cell lines are especially challenging to transfect. jetMESSENGER® gives high transfection efficiency in all of these usually difficult to transfect cells, by allowing highly efficient mRNA transfection.

mRNA transfection is as easy as DNA transfection, with the advantage that mRNA does not need to reach the cell nucleus for expression nor require cell division for efficient gene expression. Hence, cells that are slow dividing or that have developed specific mechanisms to protect their genome can finally be used for gene expression.

Unravel the full potential of your cells by switching to mRNA transfection.

Polyplus-transfection - Trends in Transfection A video presenting mRNA transfection for hard-to-transfect cells is available

Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA (Jove)

Ordering information

Reference NumberAmount of reagentAmount of buffer
101000056jetMESSENGER® 0.1 mL10 ml
101000005jetMESSENGER® 0.75 mL60 ml

1.5 ml is sufficient to perform ca.375 transfections in 6-well plates. Bulk quantities are available upon request. Please contact us.

Request a quote


Outperforms DNA transfection every time

jetMESSENGER® is a highly efficient and gentle mRNA transfection reagent. mRNA transfection presents many advantages versus DNA transfection:

  • no risk of genome integration, hence no genome modification of the transfected cell
  • no promoter regulation issue
  • no need to reach the nucleus for efficient expression
  • more gentle process

jetMESSENGER® has been specifically designed to offer outstanding transfection efficiency in cells that are usually hard-to-transfect, finally allowing relevant gene expression experiments in almost all cell types (Fig. 1).

jetMESSENGER - mRNA - DNA comparison

Fig. 1. jetMESSENGER® outperforms its main DNA transfection reagent competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection of eGFP mRNA (5meC, pseudo-uridine, Trilink™) or plasmid DNA encoding for eGFP. Conditions were used according to the manufacturers’ recommendation.

Allows higher gene expression than direct competitors

jetMESSENGER® is extremely efficient especially in hard-to-transfect cells compared to other mRNA transfection reagents (Fig. 2.).

jetMESSENGER - mRNA comparison competitors

Fig. 2. Higher transfection efficiency using jetMESSENGER® compared to its main competitor. Transfections were performed in various hard-to-transfect cell lines with eGFP mRNA (5meC, pseudo-uridine, Trilink™) using jetMESSENGER® or Lipofectamine® MessengerMAX™. Conditions were used according to the manufacturers’ recommendation. 24 h post-transfection, transfection efficiency was assessed by FACS analysis.

Works on a variety of cell lines

With jetMESSENGER®, achieve outstanding transfection efficiency in a wide variety of cell lines, from adherent and suspension cancer cells, to sensitive primary cells and neurons. jetMESSENGER® is also perfectly suitable for reprogramming experiments in fibroblasts and stem cells (Table 1).

Table 1. jetMESSENGER® leads to high transfection efficiency in a wide variety of cell types.

Easy to use

mRNA transfection is as easy as DNA transfection: just dilute the mRNA in the provided buffer, add jetMESSENGER® to the diluted mRNA and add the mix to the cells’ growth medium in presence/absence of serum (compatible with antibiotics) (Fig. 3).

jetMESSENGER - Protocol

Fig. 3. Easy transfection process

Extremely gentle to cells

By eliminating the need to reach the nucleus for efficient expression, jetMESSENGER® allows transfection of quiescent and slow dividing cells. In addition, jetMESSENGER® operates through an extremely gentle process. Cell viability remains extremely high during transfection and cell morphology is maintained (Fig. 4). Make your transfection experiments physiologically relevant!

jetMESSENGER - Microscopy

Fig. 4. jetMESSENGER® provides a better cell viability and a higher protein expression than DNA transfection. Primary Rat Cortical neurons, Hep G2 and mouse stem cells were analyzed 48 h after transfection using phase contrast and fluorescent microscopy. The transfections were performed with jetMESSENGER®/eGFP mRNA (5meC, pseudo-uridine, Trilink™) and L2K/eGFP plasmid DNA according to the manufacturers’ recommendations for each reagent.


If you have any questions regarding jetMESSENGER®, please visit our dedicated Frequently asked questions or contact us at support@polyplus-transfection.com.

To learn more about mRNA synthesis, follow the link below:

Learn more about RNA Synthesis


mRNA transfection

jetMESSENGER® is perfectly suited for gene expression in difficult to transfect cells such as neurons, primary cellsstem cells and various cancer cell lines, through mRNA transfection (Fig. 1 & Fig. 2).

📰 Read Application note: Enhanced non-viral gene delivery to stem cells with jetMESSENGER®


jetMESSENGER - Cells

Fig. 1. jetMESSENGER®-mediated transfection in different hard-to-transfect cells. Various cell lines were analyzed by fluorescent microscopy 48 h after transfection. Transfections were performed with jetMESSENGER®/eGFP mRNA (5meC, pseudo-uridine, Trilink™) according to the manufacturer’s recommendation. Data on OVCAR-3, NCCIT, MDA-MB-231 & SW1353 cell lines are kindly provided by Inserm U1199 « BioTICLA » (Caen, France).

jetMESSENGER - Primary mouse lung endothelial cells

Fig. 2. jetMESSENGER® is suitable to transfect mRNAs in primary mouse lung engothelial cells. Primary lung cells were analyzed by fluorescent microscopy 48 hours post transfection. Cotransfection was performed with modified RNAs for nuclear GFP and mCherry using jetMESSENGER®. Data are kindly provided by T. Itkin, Weill Cornell Medicine, USA.

Neurology applications

📰 Read Application note: Efficient transient gene expression in neurosphere with jetMESSENGER®

📰 Read Application note: Efficient Gene Delivery to Neuronal Cells with jetMESSENGER®

jetMESSENGER® leads to high transfection efficiency in primary neurons, while maintaining a physiological and healthy cell morphology for several days, allowing long term expression for neurology applications (Fig. 3.).

jetMESSENGER - Neuron brightfield JetMESSENGER - Neuron fluorescence

Fig. 3. jetMESSENGER® is suitable to transfect mRNA in primary rat cortical neurons. Primary rat cortical neurons were analyzed by bright-field and fluorescent microscopy 48 h after transfection. Transfection was performed with jetMESSENGER®/eGFP mRNA (5meC, pseudo-uridine, Trilink™) according to the manufacturer’s recommendations.

CRISPR/Cas9 applications

jetMESSENGER® is perfectly suited for RNA-based genome editing using CRISPR/Cas9 .

Cell reprogramming

Cells transfected with jetMESSENGER® show a high viability and a long term expression of the protein of interest, making jetMESSENGER® the ideal transfection reagent for reprogramming studies. It is now possible to turn any cell of the body into pluripotent stem cells. For example, it is possible to use mRNA transfection with jetMESSENGER® to transform fibroblasts into IPs.

jetMESSENGER - Fibroblast brightfield jetMESSENGER - Fibroblast fluorescence

Fig. 4. jetMESSENGER® is suitable to transfect mRNA in BJ cells (human fibroblast). BJ cells were analyzed by bright-field and fluorescent microscopy 48 h after transfection. Transfection was performed with jetMESSENGER®/eGFP mRNA (5meC, pseudo-uridine, Trilink™) according to the manufacturer’s recommendations.


Every batch of jetMESSENGER® mRNA transfection reagent is tested in-house by mRNA transfection of CaCo-2 cells with a GFP-expressing mRNA and each vial of reagent is provided with a Certificate of Analysis.


"After some optimization done with the help of Polyplus-transfection Scientific Support, I obtained with jetMESSENGER® an excellent transfection efficiency while maintaining a good viability in primary NK cells and NK cell lines."
 Nabil L, Pharmaceutical company, France. 
“We observed outstanding transfection efficiency with jetMESSENGER in our Human Adrenal Gland cells; over 80% gene editing where Messenger-MAX and Stem-MAX maxed out at 45-50%. Bravo!”
 Y. N, Vervetx 
“We were very happy with the performance of jetMESSENGER and in vivo-jetRNA in delivering our antigen-expressing mRNA to a variety of cells in vitro and in vivo. The response achieved in relation to dose exceeded our expectations, without any observable adverse effect from the formulation.”
 Remi C., Columbia University, New York, NY 
“Application of jetMESSENGER reagent to introduce modified RNA molecules into primary lung mouse endothelial cells (a cell population which is generally hard-to-transfect with DNA/RNA using non-viral methodology) resulted with the highest transfection rate (>70%) and with the lowest toxicity effects as compared to other tested RNA/DNA transfection reagents (jetPEI, TransIT, & RNAiMAX).”
 Itkin T., Weill Cornell Medicine, New York, United States 
“jetMESSENGER appears as a very effective reagent for RNA transfection in human cancer cells of various origins. The expression level intensity and the transfected cells proportion were very impressive as compared to conventional DNA transfection reagents, without any sign of cytotoxicity.”
 Brotin E., Abeilard E., Denoyelle C., Poulain L. Inserm U1199 « BioTICLA », Caen, France 
“The protocol and handling of jetMESSENGER Kit was very simple and easy to use in comparison to other mRNA kits we tested. In our hands, jetMESSENGER is the only component that resulted in 60% transfection efficiency in peritoneal macrophages, in less than 9h while not affecting cell viability.”
 Herb M., University of Cologne, Germany 
“The jetMESSENGER kit makes mRNA transfections very easy. The one step protocol is easy to use and can be combined with media comprising serum. It is a great tool!”
 Pollet J., Baylor College of Medicine (BCM), USA 
“The experiments I did were really good, the transfection worked greatly and I did also a comparison with another transfection kit from a competitor, I have to say yours is much better.”
 Stefano C., Baseclick GmbH, Germany 
“I obtained a very good transfection efficiency using jetMESSENGER®. It is easy to use and the protocol is well described."
 Kathleen S., Pharmaceutical company, Germany 


Order by :  
Found 40 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
Ouranidis A. et al. (2021)

Vaccines (Basel) 9, 890
Biopharmaceutics 4.0, Advanced Pre-Clinical Development of mRNA-Encoded Monoclonal Antibodies to Immunosuppressed Murine Models
More details
Porter RL. et al. (2022)

, J Clin Invest
Satellite repeat RNA expression in epithelial ovarian cancer associates with a tumor immunosuppressive phenotype
More details
K-562in vitromRNAjetMESSENGER
Liaud, N. et al. (2019)

PLoS Genet 15, e1008057
Cellular response to small molecules that selectively stall protein synthesis by the ribosome
More details
HAP1in vitrolncRNAjetMESSENGER
Yoneda R. et al. (2021)

Int J Mol Sci 22, 11014
m 6 A Modified Short RNA Fragments Inhibit Cytoplasmic TLS/FUS Aggregation Induced by Hyperosmotic Stress
More details
Rat primary Schwann cellsin vitromRNAjetMESSENGER
Puhl DL. et al. (2023)

Acta Biomater 155, 370-385
Electrospun fiber-mediated delivery of neurotrophin-3 mRNA for neural tissue engineering applications
More details
Mouse bone marrow-derived macrophages, Mouse peritoneal macrophagesin vitromRNAjetMESSENGER
Herb M. et al. (2020)

J Vis Exp ,
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA
More details
Jurkatin vitromRNAjetMESSENGER
Kheirolomoom A. et al. (2022)

Biomaterials 281, 121339
In situ T-cell transfection by anti-CD3-conjugated lipid nanoparticles leads to T-cell activation, migration, and phenotypic shift
More details
HEK-293Tin vitromRNAjetMESSENGER
Kicmal TM. et al. (2019)

J Virol 93, e01054-19
Polyamine Depletion Abrogates Enterovirus Cellular Attachment
More details
C8-D1A [Astrocyte type I clone]in vitromRNAjetMESSENGER
Zhang N. et al. (2022)

Front Immunol 13, 838990
JEV Infection Induces M-MDSC Differentiation Into CD3+ Macrophages in the Brain
More details
Viegas, C. S. B. et al. (2017)

PLoS One 12, e0177829
Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases
More details