Overview
Specifications
| Reagent | jetMESSENGER® |
|---|---|
| Molecule delivered | mRNA |
| Applications | Gene expression |
| Cell types | Hard-to-transfect cells |
| Number of transfections | 1.5 ml of jetMESSENGER® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates |
| Storage | Store jetMESSENGER® at 5 °C ± 3°C. |
| Provided with | mRNA buffer |
Summary
Take your gene expression to the next level by switching to mRNA!
Primary cells, neurons, suspension cells and various cancer cell lines are especially challenging to transfect. jetMESSENGER® gives high transfection efficiency in all of these usually difficult to transfect cells, by allowing highly efficient mRNA transfection.
mRNA transfection is as easy as DNA transfection, with the advantage that mRNA does not need to reach the cell nucleus for expression nor require cell division for efficient gene expression. Hence, cells that are slow dividing or that have developed specific mechanisms to protect their genome can finally be used for gene expression.
Unravel the full potential of your cells by switching to mRNA transfection.
A video presenting mRNA transfection for hard-to-transfect cells is available
Highly Efficient Transfection of Primary Macrophages with In Vitro Transcribed mRNA (Jove)
Ordering information
| Reference Number | Amount of reagent | Amount of buffer |
|---|---|---|
| 101000056 | jetMESSENGER® 0.1 mL | 10 ml |
| 101000005 | jetMESSENGER® 0.75 mL | 60 ml |
Description
Outperforms DNA transfection every time
jetMESSENGER® is a highly efficient and gentle mRNA transfection reagent. mRNA transfection presents many advantages versus DNA transfection:
- no risk of genome integration, hence no genome modification of the transfected cell
- no promoter regulation issue
- no need to reach the nucleus for efficient expression
- more gentle process
jetMESSENGER® has been specifically designed to offer outstanding transfection efficiency in cells that are usually hard-to-transfect, finally allowing relevant gene expression experiments in almost all cell types (Fig. 1).
Fig. 1. jetMESSENGER® outperforms its main DNA transfection reagent competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection of eGFP mRNA (5meC, pseudo-uridine, Trilink™) or plasmid DNA encoding for eGFP. Conditions were used according to the manufacturers’ recommendation.
Allows higher gene expression than direct competitors
jetMESSENGER® is extremely efficient especially in hard-to-transfect cells compared to other mRNA transfection reagents (Fig. 2.).
Fig. 2. Higher transfection efficiency using jetMESSENGER® compared to its main competitor. Transfections were performed in various hard-to-transfect cell lines with eGFP mRNA (5meC, pseudo-uridine, Trilink™) using jetMESSENGER® or Lipofectamine® MessengerMAX™. Conditions were used according to the manufacturers’ recommendation. 24 h post-transfection, transfection efficiency was assessed by FACS analysis.
Works on a variety of cell lines
With jetMESSENGER®, achieve outstanding transfection efficiency in a wide variety of cell lines, from adherent and suspension cancer cells, to sensitive primary cells and neurons. jetMESSENGER® is also perfectly suitable for reprogramming experiments in fibroblasts and stem cells (Table 1).
Table 1. jetMESSENGER® leads to high transfection efficiency in a wide variety of cell types.
Easy to use
mRNA transfection is as easy as DNA transfection: just dilute the mRNA in the provided buffer, add jetMESSENGER® to the diluted mRNA and add the mix to the cells’ growth medium in presence/absence of serum (compatible with antibiotics) (Fig. 3).
Fig. 3. Easy transfection process
Extremely gentle to cells
By eliminating the need to reach the nucleus for efficient expression, jetMESSENGER® allows transfection of quiescent and slow dividing cells. In addition, jetMESSENGER® operates through an extremely gentle process. Cell viability remains extremely high during transfection and cell morphology is maintained (Fig. 4). Make your transfection experiments physiologically relevant!
Fig. 4. jetMESSENGER® provides a better cell viability and a higher protein expression than DNA transfection. Primary Rat Cortical neurons, Hep G2 and mouse stem cells were analyzed 48 h after transfection using phase contrast and fluorescent microscopy. The transfections were performed with jetMESSENGER®/eGFP mRNA (5meC, pseudo-uridine, Trilink™) and L2K/eGFP plasmid DNA according to the manufacturers’ recommendations for each reagent.
FAQ
If you have any questions regarding jetMESSENGER®, please visit our dedicated Frequently asked questions or contact us at support@polyplus-transfection.com.
To learn more about mRNA synthesis, follow the link below:
Applications
mRNA transfection
jetMESSENGER® is perfectly suited for gene expression in difficult to transfect cells such as neurons, primary cells, stem cells and various cancer cell lines, through mRNA transfection (Fig. 1 & Fig. 2).
📰 Read Application note: Enhanced non-viral gene delivery to stem cells with jetMESSENGER®
Fig. 1. jetMESSENGER®-mediated transfection in different hard-to-transfect cells. Various cell lines were analyzed by fluorescent microscopy 48 h after transfection. Transfections were performed with jetMESSENGER®/eGFP mRNA (5meC, pseudo-uridine, Trilink™) according to the manufacturer’s recommendation. Data on OVCAR-3, NCCIT, MDA-MB-231 & SW1353 cell lines are kindly provided by Inserm U1199 « BioTICLA » (Caen, France).
Fig. 2. jetMESSENGER® is suitable to transfect mRNAs in primary mouse lung engothelial cells. Primary lung cells were analyzed by fluorescent microscopy 48 hours post transfection. Cotransfection was performed with modified RNAs for nuclear GFP and mCherry using jetMESSENGER®. Data are kindly provided by T. Itkin, Weill Cornell Medicine, USA.
Neurology applications
📰 Read Application note: Efficient transient gene expression in neurosphere with jetMESSENGER®
📰 Read Application note: Efficient Gene Delivery to Neuronal Cells with jetMESSENGER®
jetMESSENGER® leads to high transfection efficiency in primary neurons, while maintaining a physiological and healthy cell morphology for several days, allowing long term expression for neurology applications (Fig. 3.).
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Fig. 3. jetMESSENGER® is suitable to transfect mRNA in primary rat cortical neurons. Primary rat cortical neurons were analyzed by bright-field and fluorescent microscopy 48 h after transfection. Transfection was performed with jetMESSENGER®/eGFP mRNA (5meC, pseudo-uridine, Trilink™) according to the manufacturer’s recommendations.
CRISPR/Cas9 applications
jetMESSENGER® is perfectly suited for RNA-based genome editing using CRISPR/Cas9 .
Cell reprogramming
Cells transfected with jetMESSENGER® show a high viability and a long term expression of the protein of interest, making jetMESSENGER® the ideal transfection reagent for reprogramming studies. It is now possible to turn any cell of the body into pluripotent stem cells. For example, it is possible to use mRNA transfection with jetMESSENGER® to transform fibroblasts into IPs.
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Fig. 4. jetMESSENGER® is suitable to transfect mRNA in BJ cells (human fibroblast). BJ cells were analyzed by bright-field and fluorescent microscopy 48 h after transfection. Transfection was performed with jetMESSENGER®/eGFP mRNA (5meC, pseudo-uridine, Trilink™) according to the manufacturer’s recommendations.
Quality
Every batch of jetMESSENGER® mRNA transfection reagent is tested in-house by mRNA transfection of CaCo-2 cells with a GFP-expressing mRNA and each vial of reagent is provided with a Certificate of Analysis.
Testimonials
Protocol
In order to download a product protocol or a certificate of analysis, please create an account on Polyplus ® Portal .
Why would you need to create an account?
In this personal area you will have access to:
- Product Protocols
- Certificates of Analysis
- Exclusive webinars/articles
- And surprise features!
Other files
Related blog posts
Bibliography
| Cell Line | in vitro in vivo | Delivered Molecule | Reagent | Results & Citations | |
|---|---|---|---|---|---|
| MEF | in vitro | miRNA | jetMESSENGER | Mandal P. et al. (2020) Biomedicines 8, 485 Exosome-Mediated Differentiation of Mouse Embryonic Fibroblasts and Exocrine Cells into β-Like Cells and the Identification of Key miRNAs for Differentiation | More details |
| DC2.4 | in vitro | mRNA | jetMESSENGER | Shi Y. et al. (2022) Theranostics 12, 3488-3502 Optimized mobilization of MHC class I- and II- restricted immunity by dendritic cell vaccine potentiates cancer therapy | More details |
| MEF, Mouse bone marrow-derived macrophages, Mouse peritoneal macrophages | in vitro | mRNA | jetMESSENGER | Herb, M. et al. (2019) Science Signaling , Mitochondrial reactive oxygen species enable proinflammatory signaling through disulfide linkage of NEMO | More details |
| Mouse bone marrow-derived macrophages | in vitro | mRNA | jetMESSENGER | Zhang H. et al. (2022) Front Cell Dev Biol 9, 782427 5-Hydroxymethylfurfural Alleviates Inflammatory Lung Injury by Inhibiting Endoplasmic Reticulum Stress and NLRP3 Inflammasome Activation | More details |
| Bone marrow-derived dendritic cells, DC2.4 | in vitro | mRNA | jetMESSENGER | Kliesch L. et al. (2022) Pharmaceutics 14, 2675 Lipid-Polymer Hybrid Nanoparticles for mRNA Delivery to Dendritic Cells: Impact of Lipid Composition on Performance in Different Media | More details |
| HeLa | in vitro | lncRNA | jetMESSENGER | Yoneda R. et al. (2020) J Biol Chem 295, 5626-5639 Long noncoding RNA pncRNA-D reduces cyclin D1 gene expression and arrests cell cycle through RNA m6A modification | More details |
| ex vivo porcine skin model | in vitro | mRNA | in vivo-jetPEI, jetMESSENGER | Darade AR. et al. (2022) Pharmaceutics 14, 151 Effect of mRNA Delivery Modality and Formulation on Cutaneous mRNA Distribution and Downstream eGFP Expression | More details |
| HEK-293T, RD, VERO, VERO E6 | in vitro | DNA, mRNA | jetMESSENGER, jetPEI | Choi WS. et al. (2023) Microbiol Spectr , e0316722 Development of a Universal Cloning System for Reverse Genetics of Human Enteroviruses | More details |
| CHO-K1 | in vitro | mRNA | jetMESSENGER | Ouranidis A. et al. (2021) Vaccines (Basel) 9, 890 Biopharmaceutics 4.0, Advanced Pre-Clinical Development of mRNA-Encoded Monoclonal Antibodies to Immunosuppressed Murine Models | More details |
| THP-1 | in vitro | mRNA | jetMESSENGER | Porter RL. et al. (2022) , J Clin Invest Satellite repeat RNA expression in epithelial ovarian cancer associates with a tumor immunosuppressive phenotype | More details |