INTERFERin®
siRNA, miRNA and other oligonucleotides
- Great silencing using as little as 1 nM siRNA
- Over 90% gene silencing in a wide variety of cells
- Suitable for transfection miRNA and other oligonucleotides
- Gentle mode of action for more robust data and excellent cell viability
- Compatible with serum and antibiotics
Specifications
| Reagent | INTERFERin® |
|---|---|
| Molecule delivered | siRNA, miRNA (microRNA), and other oligonucleotides: pre-miRNA, mimic miRNA, antimiR… |
| Applications | Transfection of siRNA and other oligonucleotides |
| Cell types | Adherent and suspension cells |
| Number of transfections | 1 ml of INTERFERin® is sufficient to perform 500-1000 transfections in 24-well plates. |
| Storage | 5°C ± 3°C, stable for 6 months (409-01) to at least one year (other packaging sizes) when stored appropriately |
Summary
INTERFERin® provides very high silencing efficiency already at 1 nM siRNA and can be used in a wide variety of adherent and suspension cells. Using low concentration of siRNA avoids off-target effects, and its gentle mode of action ensure more robust data and excellent cell viability. Easy to use thanks to its compatibility with serum and antibiotics, INTERFERin® is also perfectly suited for transfection of miRNA and other oligonucleotides like pre-miRNA, mimic miRNA, antimiR….
Ordering information
| Amount of reagent | Reference Number |
|---|---|
| 0.1 ml | 409-01 |
| 1 ml | 409-10 |
| 5 x 1 ml | 409-50 |
Less siRNA, less off-target effects
Several publications show that transfecting low siRNA concentrations avoids off-target effects. Indeed, these unwanted non specific side-effects are observed when transfecting siRNA at high concentrations (1,2). Hence, the reliability of the experimental data can be increased by using as little siRNA as possible. This is why INTERFERin® has been specifically designed to provide high silencing efficiency using low siRNA concentrations.
INTERFERin®-mediated delivery of 1 nM of a specific siRNA shows selective and highly efficient knockdown of gene expression, while a competitor (L2K) needs at least 10 nM siRNA to reach 50% silencing efficiency (Fig. 1).
Amazingly, 50% silencing is still achieved at 10 pM siRNA (Fig. 2).
Transfection of 1 nM siRNA targeting endogenous lamin A/C with INTERFERin® drastically reduces lamin gene expression to barely detectable level (Fig. 3).
1. Birmingham, A. et al., (2006) Nature Methods, Vol.3, No3, 199-204
2. Caffrey, D. et al., (2011) PLoS One. 2011;6(7):e21503. Epub 2011 Jul 5.
Suitable for miRNA transfection
Transfection of microRNA (miRNA) oligonucleotides is increasingly being used to analyze biological effects of specific miRNAs on cell function. INTERFERin® is the reagent of choice for delivering miRNA, miRNA mimics or pre-miRNAs.
INTERFERin® is the latest generation siRNA & miRNA transfection reagent, especially designed for high transfection efficiency in a wide variety of cells, resulting in high gene silencing or stimulation of gene expression. Indeed, some miRNA are also known to induce gene expression by association with the promoter of the gene of interest. For example, INTERFERin®-mediated delivery of miR-373 leads to a 3 fold increase in E-Cadherin expression (Fig. 4).
The cationic components of INTERFERin® require very little oligonucleotide amount, resulting in a gentle transfection process. In addition, the easy and robust protocol ensures reproducible results. Oligonucleotides like miRNA can be transfected with small quantities of INTERFERin® resulting in strong gene silencing or stimulation of gene expression. Whatever the concentration of INTERFERin® used, the effect stays approximately the same making INTERFERin® an economical reagent. For example, the fold change using different volume of INTERFERin® is always between 1.5 and 1.8 (Fig. 5).
High transfection efficiency associated with high cell viability makes INTERFERin® the reagent of choice for generating relevant data for scientific publications. Indeed, the number of publications using INTERFERin® for miRNA and miRNA related molecules has been exponentially growing since its launch (Fig. 6).
Over 90 % gene silencing
For many adherent cell lines or primary cells, 1 nM siRNA is sufficient to obtain more than 90 % gene silencing. For suspension cell lines, 80 % silencing can still be reached by INTERFERin® using 5 nM siRNA (Table 1).
Specific conditions for various cell lines are available in our Polyplus-transfection Database.
Excellent cell viability
When it comes to cell viability, INTERFERin® outperforms other transfection reagents. 48 h after transfection with 1 nM siRNA, cells transfected with INTERFERin® appear healthy, while toxicity is clearly observed with reagent S (Fig. 7).
Easy standard protocol
INTERFERin® is ready to use and the protocol is straightforward. A starting concentration of 1 nM siRNA ensures silencing of most genes in most cell types (Fig. 8).
INTERFERin® is compatible with both serum and antibiotics, hence avoiding any time consuming washes and medium changes; furthermore INTERFERin® can be left on the cells without any adverse effects.
Take a look at our Expert Tips in Genetic Engineering and Biotechnology News .
VIDEO: siRNA transfection using INTERFERin®
FAQ
If you have any questions regarding INTERFERin®, please visit our dedicated Frequently asked questions or contact us at support@polyplus-transfection.com.
Our INTERFERin® reagent is perfectly suited to perform RNA interference experiments, which correspond to the specific inhibition of gene expression promoted by siRNA. siRNAs are gene specific complementary double-stranded RNA oligonucleotides present in the cytoplasm of plants, worms and mammalian cells, and can be efficiently transfected into cells with INTERFERin®.
Here is a selection of relevant references using INTERFERin®, more are available in our Polyplus-transfection Database.
Carduner, L., Picot, C. R., Leroy-Dudal, J., Blay, L., Kellouche, S., Carreiras, F. (2014). Cell cycle arrest or survival signaling through alphav integrins, activation of PKC and ERK1/2 lead to anoikis resistance of ovarian cancer spheroids., Exp Cell Res 320, 329-4.
Hu, B., Castillo, E., Harewood, L., Ostano, P., Reymond, A., Dummer, R., Raffoul, W., Hoetzenecker, W., Hofbauer, G. F., Dotto, G. P. (2012).Multifocal epithelial tumors and field cancerization from loss of mesenchymal CSL signaling., Cell 149, 1207.
Lai, L., Song, Y., Liu, Y., Chen, Q., Han, Q., Chen, W., Pan, T., Zhang, Y., Cao, X., Wang, Q. (2013). MicroRNA-92a negatively regulates Toll-like receptor (TLR)-triggered inflammatory response in macrophages by targeting MKK4 kinase., J Biol Chem 288, 7956.
Nilsson, R., Jain, M., Madhusudhan, N., Sheppard, N. G., Strittmatter, L., Kampf, C., Huang, J., Asplund, A., Mootha, V. K. (2014). Metabolic enzyme expression highlights a key role for MTHFD2 and the mitochondrial folate pathway in cancer., Nat Commun 5, 3128.
Qin, W., Shi, Y., Zhao, B., Yao, C., Jin, L., Ma, J., Jin, Y. (2010). MiR-24 regulates apoptosis by targeting the open reading frame (ORF) region of FAF1 in cancer cells., PLoS One 5, e9429.
“I prefer INTERFERin [vs two competitors]. Awesome product with very competitive price.” – Latonia T.S., Winship Cancer Institute of Emory University, United States
“Cells seemed to be less stressed compared to [competitor]. I observed less apoptotic cells and will order INTERFERin for my next experiments. Thanks again for the trial sample. Best wishes, Anja” – Anja B., Goethe Universität Frankfurt, Germany
“I use your INTERFERin siRNA reagent in primary human Macrophages with terrific results.” – Jason H., Emory University, United States
“This is far better than some other well known products in transfecting siRNA.” – Venkateswarlu K., Swansea University, United Kingdom
Every batch of INTERFERin® is tested in house in a transfection assay on A549-Luc cells, constitutively expressing the Luciferase gene. The silencing efficiency obtained using 1 nM siRNA and INTERFERin® for each batch is indicated on the Certificate of Analysis.
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