Overview
Specifications
| Reagent | in vivo-jetPEI®-Man |
|---|---|
| Molecule delivered | DNA |
| Applications | in vivo functional studies |
| Targeted organs | Improved delivery to mannose-receptor expressing tissue |
| Injection routes | Systemic delivery (Tail vein, intravenous, intraperitoneal injection) |
| Number of transfections | 100 µl of in vivo-jetPEI®-Man delivery reagent is sufficient to perform 15 to 25 intravenous injections in mouse. |
| Storage | -20 °C, for at least 12 months |
| Provided with | 10% glucose solution |
Summary
in vivo-jetPEI® is a powerful polymer based reagent used to deliver any kind of nucleic acid to target a wide range of organs and tissues in animals. in vivo-jetPEI® ligand-conjugated derivatives have been designed to enhance delivery to cell expressing specific receptors.
in vivo-jetPEI®-Man is a mannose-conjugated in vivo-jetPEI® designed to enhance delivery to cells expressing mannose-specific membrane receptors, such as macrophages and dendritic cells.
Ordering information
| Reference Number | Amount of reagent | Amount of 10% glucose solution |
|---|---|---|
| 203-10G | 0.1 ml | 10 ml |
Description
Suitable for in vivo delivery of DNA, siRNA, miRNA, oligonucleotides and mRNA
in vivo-jetPEI®-Man is the reagent of choice to deliver any type of nucleic acid to mediate gene expression or gene silencing in various tissues, with enhanced targeting to macrophages and dendritic cells.
in vivo-jetPEI®-Man is a versatile reagent as it is suitable for the delivery of plasmid DNA, siRNA, shRNA, miRNA, oligonucleotides and mRNA.
Easy two-step protocol
in vivo-jetPEI®-Man is a very easy-to-use delivery reagent. The protocol consists in preparing the nucleic acid and reagent separately in 5% glucose solution, mixing them together and then, after a 15 min incubation time at room temperature, injecting the nucleic acid/ in vivo-jetPEI®-Man complexes formed into animal (Fig. 1 ).
Fig. 1: in vivo-jetPEI-Man – Protocol
Suitable for any animal model
in vivo-jetPEI®-Man is ideal for nucleic acid delivery in mice. However, the protocol is so easy and versatile that it can be adapted to other species.
Our delivery experts are available to adapt our protocol to your animal model and provide you with starting conditions for your application (Please contact the Scientific Support).
Safe: no inflammatory response triggered
in vivo-jetPEI®-Man offers a reliable and safe alternative to viral vectors as it does not induce any significant inflammatory response.
Applications
in vivo functional studies
in vivo-jetPEI®-Man is perfectly suited to study gene function in macrophages and dendritic cells in vivo and provides the easiest method for the validation into animals of in vitro functional studies.
Vaccination/immunization
in vivo administration of plasmid DNA formulated with in vivo-jetPEI®-Man can elicit the induction of a robust and persistent immune response, hence protecting animals from different viruses or pathogens challenge.
Citations
Here is a selection of relevant references using in vivo-jetPEI®-Man, more are available in the Bibliography tab.
Lisziewicz, J., Trocio, J., Whitman, L., Varga, G., Xu, J., Bakare, N., Erbacher, P., Fox, C., Woodward, R., Markham, P., Arya, S., Behr, J. P., Lori, F. (2005). DermaVir: a novel topical vaccine for HIV/AIDS., J Invest Dermatol 124, 160-9.
Lisziewicz, J., Trocio, J., Xu, J., Whitman, L., Ryder, A., Bakare, N., Lewis, M. G., Wagner, W., Pistorio, A., Arya, S., Lori, F. (2005). Control of viral rebound through therapeutic immunization with DermaVir., Aids 19, 35-43.
Lisziewicz, J., Kelly, L., Lori, F. (2006). Topical DermaVir vaccine targeting dendritic cells., Curr Drug Deliv 3, 83-8.
Lorincz, O., Toke, E. R., Somogyi, E., Horkay, F., Chandran, P. L., Douglas, J. F., Szebeni, J., Lisziewicz, J. (2012). Structure and biological activity of pathogen-like synthetic nanomedicines., Nanomedicine 8, 497-5.
Smitha, S., Raina, O. K., Singh, B. P., Samanta, S., Velusamy, R., Dangoudoubiyam, S., Tripathi, A., Gupta, P. K., Sharma, B., Saxena, M. (2010). Immune responses to polyethylenimine-mannose-delivered plasmid DNA encoding a Fasciola gigantica fatty acid binding protein in mice., J Helminthol 84, 149-5.
Quality
Polyplus-transfection® is ISO 9001 Quality Management System accredited since 2002; this level of certification assures global customers that the supplier has established reliable and effective processes for product development, manufacturing, sales and customer support.
Each lot of in vivo-jetPEI®-Man is validated by DNA (pCMV-Luciferase) delivery into mouse through intravenous injection. The amount of luciferase (ng) per mg of protein is indicated on the certificate of analysis for each lot of reagent. Moreover, absence of endotoxin is verified in every lot of in vivo-jetPEI®-Man.
Bibliography
| Cell Line | in vitro in vivo | Delivered Molecule | Reagent | Results & Citations | |
|---|---|---|---|---|---|
| - | in vivo | DNA | in vivo-jetPEI-Man | Lisziewicz, J., Kelly, L., Lori, F. (2006) Curr Drug Deliv 3, 83-8 Topical DermaVir vaccine targeting dendritic cells | More details |
| - | in vivo | DNA | in vivo-jetPEI-Man | Lisziewicz, J., Trocio, J., Whitman, L., Varga, G., Xu, J., Bakare, N., Erbacher, P., Fox, C., Woodward, R., Markham, P., Arya, S., Behr, J. P., Lori, F. (2005) J Invest Dermatol 124, 160-9 DermaVir: a novel topical vaccine for HIV/AIDS | More details |
| - | in vivo | DNA | in vivo-jetPEI-Man | Lisziewicz, J., Trocio, J., Xu, J., Whitman, L., Ryder, A., Bakare, N., Lewis, M. G., Wagner, W., Pistorio, A., Arya, S., Lori, F. (2005) Aids 19, 35-43 Control of viral rebound through therapeutic immunization with DermaVir | More details |
| - | in vivo | DNA | in vivo-jetPEI-Man | Lorincz, O., Toke, E. R., Somogyi, E., Horkay, F., Chandran, P. L., Douglas, J. F., Szebeni, J., Lisziewicz, J. (2012) Nanomedicine 8, 497-506 Structure and biological activity of pathogen-like synthetic nanomedicines | More details |
| - | in vivo | DNA | in vivo-jetPEI-Man | Smitha, S., Raina, O. K., Singh, B. P., Samanta, S., Velusamy, R., Dangoudoubiyam, S., Tripathi, A., Gupta, P. K., Sharma, B., Saxena, M. (2010) J Helminthol 84, 149-55 Immune responses to polyethylenimine-mannose-delivered plasmid DNA encoding a Fasciola gigantica fatty acid binding protein in mice | More details |
| - | in vivo | shRNA oligonucleotide | in vivo-jetPEI-Man | Furuya, H., Tamashiro, P. M., Shimizu, Y., Iino, K., Peres, R., Chen, R., Sun, Y., Hannun, Y. A., Obeid, L. M., Kawamori, T. (2017) Carcinogenesis 38, 1218-1227 Sphingosine Kinase 1 expression in peritoneal macrophages is required for colon carcinogenesis | More details |