Overview

Specifications

Reagent

in vivo-jetPEI®-Man

Molecule delivered

DNA
siRNA
miRNA
oligonucleotides
mRNA

Applications

in vivo functional studies
Vaccination/immunization

Targeted organs

Improved delivery to mannose-receptor expressing tissue

Injection routes

Systemic delivery (Tail vein, intravenous, intraperitoneal injection)
Local injection

Number of transfections

100 µl of in vivo-jetPEI®-Man delivery reagent is sufficient to perform 15 to 25 intravenous injections in mouse.

Storage

-20 °C, for at least 12 months

Provided with

10% glucose solution


Summary

in vivo-jetPEI®  is a powerful polymer based reagent used to deliver any kind of nucleic acid to target a wide range of organs and tissues in animals. in vivo-jetPEI® ligand-conjugated derivatives have been designed to enhance delivery to cell expressing specific receptors.
in vivo-jetPEI®-Man is a mannose-conjugated in vivo-jetPEI® designed to enhance delivery to cells expressing mannose-specific membrane receptors, such as macrophages and dendritic cells.

Ordering information

Reference NumberAmount of reagentAmount of 10% glucose solution
203-10G0.1 ml10 ml

Description

Suitable for in vivo delivery of DNA, siRNA, miRNA, oligonucleotides and mRNA

in vivo-jetPEI®-Man is the reagent of choice to deliver any type of nucleic acid to mediate gene expression or gene silencing in various tissues, with enhanced targeting to macrophages and dendritic cells.

in vivo-jetPEI®-Man is a versatile reagent as it is suitable for the delivery of plasmid DNA, siRNA, shRNA, miRNA, oligonucleotides and mRNA.

Easy two-step protocol

in vivo-jetPEI®-Man is a very easy-to-use delivery reagent. The protocol consists in preparing the nucleic acid and reagent separately in 5% glucose solution, mixing them together and then, after a 15 min incubation time at room temperature, injecting the nucleic acid/ in vivo-jetPEI®-Man complexes formed into animal (Fig. 1 ).

Fig. 1: in vivo-jetPEI-Man – Protocol

Suitable for any animal model

in vivo-jetPEI®-Man is ideal for nucleic acid delivery in mice. However, the protocol is so easy and versatile that it can be adapted to other species.

Our delivery experts are available to adapt our protocol to your animal model and provide you with starting conditions for your application (Please contact the Scientific Support).

Safe: no inflammatory response triggered

in vivo-jetPEI®-Man offers a reliable and safe alternative to viral vectors as it does not induce any significant inflammatory response.

Applications

in vivo functional studies

in vivo-jetPEI®-Man is perfectly suited to study gene function in macrophages and dendritic cells in vivo and provides the easiest method for the validation into animals of in vitro functional studies.

Read more…

Vaccination/immunization

in vivo administration of plasmid DNA formulated with in vivo-jetPEI®-Man can elicit the induction of a robust and persistent immune response, hence protecting animals from different viruses or pathogens challenge.

Read more…

Citations

Here is a selection of relevant references using in vivo-jetPEI®-Man, more are available in the Bibliography tab.

Lisziewicz, J., Trocio, J., Whitman, L., Varga, G., Xu, J., Bakare, N., Erbacher, P., Fox, C., Woodward, R., Markham, P., Arya, S., Behr, J. P., Lori, F. (2005). DermaVir: a novel topical vaccine for HIV/AIDS., J Invest Dermatol 124, 160-9.

Lisziewicz, J., Trocio, J., Xu, J., Whitman, L., Ryder, A., Bakare, N., Lewis, M. G., Wagner, W., Pistorio, A., Arya, S., Lori, F. (2005). Control of viral rebound through therapeutic immunization with DermaVir., Aids 19, 35-43.

Lisziewicz, J., Kelly, L., Lori, F. (2006). Topical DermaVir vaccine targeting dendritic cells., Curr Drug Deliv 3, 83-8.

Lorincz, O., Toke, E. R., Somogyi, E., Horkay, F., Chandran, P. L., Douglas, J. F., Szebeni, J., Lisziewicz, J. (2012). Structure and biological activity of pathogen-like synthetic nanomedicines., Nanomedicine 8, 497-5.

Smitha, S., Raina, O. K., Singh, B. P., Samanta, S., Velusamy, R., Dangoudoubiyam, S., Tripathi, A., Gupta, P. K., Sharma, B., Saxena, M. (2010). Immune responses to polyethylenimine-mannose-delivered plasmid DNA encoding a Fasciola gigantica fatty acid binding protein in mice., J Helminthol 84, 149-5.

Quality

Polyplus-transfection® is ISO 9001 Quality Management System accredited since 2002; this level of certification assures global customers that the supplier has established reliable and effective processes for product development, manufacturing, sales and customer support.

Each lot of in vivo-jetPEI®-Man is validated by DNA (pCMV-Luciferase) delivery into mouse through intravenous injection. The amount of luciferase (ng) per mg of protein is indicated on the certificate of analysis for each lot of reagent. Moreover, absence of endotoxin is verified in every lot of in vivo-jetPEI®-Man.

Protocol

To view our protocols, please fill in the fields below and click download.

Other files

Bibliography

Order by :  
Found 6 results :
Cell Linein vitro
in vivo
Delivered MoleculeReagentResults & Citations
-in vivoDNAin vivo-jetPEI-Man
Lisziewicz, J. et al. (2006)

Curr Drug Deliv 3, 83-8
Topical DermaVir vaccine targeting dendritic cells
More details
-in vivoDNAin vivo-jetPEI-Man
Lisziewicz, J. et al. (2005)

J Invest Dermatol 124, 160-9
DermaVir: a novel topical vaccine for HIV/AIDS
More details
-in vivoDNAin vivo-jetPEI-Man
Lisziewicz, J. et al. (2005)

Aids 19, 35-43
Control of viral rebound through therapeutic immunization with DermaVir
More details
-in vivoDNAin vivo-jetPEI-Man
Lorincz, O. et al. (2012)

Nanomedicine 8, 497-506
Structure and biological activity of pathogen-like synthetic nanomedicines
More details
-in vivoDNAin vivo-jetPEI-Man
Smitha, S. et al. (2010)

J Helminthol 84, 149-55
Immune responses to polyethylenimine-mannose-delivered plasmid DNA encoding a Fasciola gigantica fatty acid binding protein in mice
More details
-in vivoshRNA oligonucleotidein vivo-jetPEI-Man
Furuya, H. et al. (2017)

Carcinogenesis 38, 1218-1227
Sphingosine Kinase 1 expression in peritoneal macrophages is required for colon carcinogenesis
More details